rbg-1 Antibody

Antibody Overview

The RBG-1 antibody (ab108318) is a rabbit monoclonal antibody developed using patented technology. It targets the BRG1 protein (SMARCA4), which has a molecular weight of ~185 kDa. Validated for use in Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry, this antibody is compatible with human, mouse, and rat samples .

Key features:

• High specificity: Validated using SMARCA4 knockout cell lines, with no cross-reactivity observed in knockout controls .

• Batch consistency: Produced in recombinant format for reliability across experiments .

• Broad application: Effective in multiple techniques, including co-staining with tubulin markers .

Western Blot Analysis

The antibody detects BRG1 at the expected molecular weight (185 kDa) in human, mouse, and rat lysates. Knockout validation confirms specificity:

Source: ab108318 validation data .

Immunohistochemistry (IHC)

Optimized protocols for formalin-fixed paraffin-embedded tissues include:

• Antigen retrieval: Tris-EDTA buffer (pH 9.0).

• Dilution: 1:500 (0.23 µg/ml).

• Secondary antibody: HRP Polymer .

Functional Studies

• Chromatin remodeling: BRG1 regulates gene expression by altering chromatin structure, impacting cancer progression and stem cell differentiation .

• Disease models: Used to investigate BRG1's role in malignancies such as lung adenocarcinoma and glioblastoma .

Technical Performance

• Co-staining: Compatible with markers like α-tubulin (Alexa Fluor® 594) .

• Sensitivity: Detects BRG1 at low concentrations, critical for rare cell populations .

References

- ab108318 validation data (Abcam).
- Rbg1-Tma46 enzymatic activity and structural analysis.
- Ribosome association and genetic interactions of Rbg1.