RBFOX2 Antibody, Biotin conjugated
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In Stock 
Description
Applications in Research
The biotin-conjugated RBFOX2 antibody is employed in diverse techniques to study RBFOX2’s cellular and molecular roles:
Western Blotting (WB)
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Purpose: Quantify RBFOX2 protein levels or validate RNA interference (RNAi) knockdown efficiency.
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Protocol:
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Observed Molecular Weight: ~47 kDa (theoretical) to 50–60 kDa (observed due to post-translational modifications) .
Immunohistochemistry (IHC)
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Purpose: Localize RBFOX2 in tissue sections (e.g., human endometrial cancer, mouse/rat brain) .
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Protocol:
Enzyme-Linked Immunosorbent Assay (ELISA)
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Purpose: Quantify RBFOX2 in lysates or recombinant protein samples.
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Protocol:
Mechanistic Roles of RBFOX2
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m⁶A Recognition and Chromatin Regulation:
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RBFOX2 binds N⁶-methyladenosine (m⁶A)-modified chromatin-associated RNAs (caRNAs), recruiting the m⁶A methyltransferase complex (MTC) to deposit m⁶A at promoter-associated RNAs (paRNAs) .
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This facilitates interaction with YTHDC1 and polycomb repressive complex 2 (PRC2), leading to transcriptional silencing .
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Splicing Regulation:
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Leukemia and Self-Renewal:
Western Blot Optimization
| Parameter | Recommendation | Source |
|---|---|---|
| Primary Antibody | 1:500–1:5000 dilution in 5% BSA/TBST | |
| Blocking | 5% non-fat milk or 3% BSA | |
| Secondary Antibody | Streptavidin-HRP (1:10,000) |
Immunostaining Tips
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Tissue Fixation: Formalin-fixed, paraffin-embedded (FFPE) sections .
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Antigen Retrieval: Required for IHC (e.g., EDTA buffer, pH 8.0) .
References and Diverse Sources
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Aviva Systems Biology: Technical details on biotin-conjugated RBFOX2 antibody (WB application) .
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Nature: RBFOX2’s role in m⁶A recognition and leukemia maintenance .
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Boster Bio: IHC/IF protocols using unconjugated RBFOX2 antibody (applicable to biotin-conjugated versions with secondary antibodies) .
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Cusabio: ELISA and IF applications for biotin-conjugated RBFOX2 antibody .
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Biocompare: Cross-referenced antibody reactivity and applications .
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Typically, we can ship products within 1-3 business days after receiving your order. Delivery times may vary depending on the purchase method or location. For specific delivery timeframes, please contact your local distributor.
Synonyms
ibody; Fox 1 homologue antibody; Fox-1 homolog B antibody; FOX2 antibody; FXH antibody; Hexaribonucleotide-binding protein 2 antibody; HNRBP2 antibody; RBFOX2 antibody; Repressor of tamoxifen transcriptional activity antibody; RFOX2_HUMAN antibody; RNA binding motif protein 9 antibody; RNA binding protein 9 antibody; RNA binding protein fox-1 homolog 2 antibody; RNA-binding motif protein 9 antibody; RNA-binding protein 9 antibody; RTA antibody
Target Names
RBFOX2
Uniprot No.
Target Background
Function
RBFOX2 is an RNA-binding protein that plays a crucial role in regulating alternative splicing events. It achieves this by binding to 5'-UGCAUGU-3' elements, thereby preventing the binding of U2AF2 to the 3'-splice site. This regulation influences alternative splicing of tissue-specific exons and differentially spliced exons during erythropoiesis. RBFOX2 also acts as a coregulatory factor for ER-alpha.
Gene References Into Functions
- A nonsense mutation in RBFOX2 has been linked to hypoplastic left heart syndrome. PMID: 27485310
- Research indicates that while RBFOX1 and RBFOX2 do not mediate neuron-specific processing of UBE3A-ATS, they play significant roles in developing neurons and are not entirely functionally redundant. PMID: 27146458
- Studies have shown that the expression patterns of these genes are indicative of the onset of EMT in in-vitro models, but not in tissue samples. However, the ratio between ESRP1 or ESRP2 and RBFOX2 significantly decreases during EMT and positively correlates with the EMT-specific phenotype in cell models. A low ESRP1/RBFOX2 ratio value is associated with an increased risk of metastasis in early breast cancer patients. PMID: 27911856
- Rbfox2 modulates the functions of vascular CaV1.2 calcium channel by dynamically regulating the expressions of alternative exons 9* and 33, which subsequently affects the vascular myogenic tone. PMID: 28993448
- Dysregulation of RBFOX2 by dominant-negative RBFOX2 is an early pathogenic event in diabetic hearts. PMID: 27239029
- RBFox2 interacts with chromatin in a nascent RNA-dependent manner. Inactivation of RBFox2 eliminates PRC2 targeting on the majority of bivalent gene promoters, leading to transcriptional de-repression. PMID: 27211866
- Some of the widespread cellular functions of Rbfox2 protein are attributed to regulation of miRNA biogenesis, and might include the mis-regulation of miR-20b and miR-107 in cancer and neurodegeneration. PMID: 27001519
- RBFOX proteins can facilitate the splicing of micro-exons. It has also been observed that PTBP1 likely regulates the inclusion of micro-exons, potentially by repressing the inclusion of micro-exons that are enhanced by RBFOX proteins and other splicing factors. [RBFOX] PMID: 25524026
- CPSF2 and SYMPK are RBFOX2 cofactors for both inclusion and exclusion of internal exons. PMID: 25921069
- RBFOX2 SNPs showed evidence for effects across multiple reading and language traits. PMID: 25065397
- Results demonstrate that the conserved Rbfox2 RNA binding protein regulates 30% of the splicing transitions observed during myogenesis and is required for the specific step of myoblast fusion. PMID: 25087874
- MBNL1 and RBFOX2 cooperate to establish a splicing program involved in pluripotent stem cell differentiation. PMID: 24048253
- RBFOX2 polymorphism is associated with breast cancer. PMID: 23143756
- RBFOX2 drives mesenchymal tissue-specific splicing in both normal and cancer tissues. PMID: 23149937
- FOX-2 is involved in splicing of ataxin-2 transcripts and this splicing event is altered by overexpression of ataxin-1. PMID: 22666429
- Functional significance of EMT-associated alternative splicing depletion of RBFOX2 in mesenchymal cells. PMID: 21876675
- This study characterizes the mechanism by which RBFOX2 regulates protein 4.1R exon 16 splicing through the downstream intronic element UGCAUG. PMID: 22083953
- The negative regulation of Rbfox2 by Rbfox3 through a novel mechanism. PMID: 21747913
- Fox-2 plays an integral role in the regulation of LH2 splicing and knockdown of Fox-2 may suggest a novel approach to strategies directed against scleroderma. PMID: 20131247
- Fox-1 and Fox-2 isoforms specifically activate splicing of neuronally regulated exons, which requires UGCAUG enhancer elements. PMID: 16260614
- Fox-1 and Fox-2 splicing factors have roles in alternative splicing of protein 4.1R. PMID: 16537540
- Fox-1/Fox-2 proteins block prespliceosome complex formation at two distinct steps through binding to two functionally important UGCAUG elements. PMID: 18573872
- These results establish hnRNP H and hnRNP F as being repressors of exon inclusion and suggest that Fox proteins enhance their ability to antagonize ASF/SF2. PMID: 18573884
- Predicting thousands of Fox-1/2 targets with conserved binding sites, at a false discovery rate of approximately 24%, including many validated experimentally, suggests a surprisingly extensive splicing regulatory network. PMID: 18794351
- These findings suggest that FOX2 functions as a critical regulator of a splicing network, and that FOX2 is important for the survival of human embryonic stem cells. PMID: 19136955
Database Links
Subcellular Location
Nucleus. Cytoplasm.
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