RBL7 Antibody

RAB7 Antibody (ab137029)

Description:

• Target: RAB7A, a GTPase involved in vesicle trafficking and lysosomal maturation .

• Host Species: Rabbit monoclonal antibody (Clone EPR7589) .

• Applications:

  • Western blot (WB) at 1:1000 dilution .

  • Immunocytochemistry/Immunofluorescence (ICC/IF) at 1:500 dilution .

  • Flow cytometry (intracellular) at 1:20 dilution .

  • Immunohistochemistry (IHC) at 1:50 dilution .

Key Validation Data:

Specificity:

• Validated in RAB7 knockout (KO) HAP1 cells, showing no cross-reactivity .

RPL7 Antibody (ab72550)

Description:

• Target: Ribosomal Protein L7, a component of the 60S ribosomal subunit .

• Host Species: Rabbit polyclonal antibody .

• Applications:

  • Western blot (WB) at 0.04 µg/mL .

  • Immunoprecipitation (IP) at 3 µg/mg lysate .

  • Immunohistochemistry (IHC-P) at 1:1000 dilution .

Key Validation Data:

Functional Role:

• Regulates translation by binding to G-rich regions in rRNA and mRNA .

Antibody Validation and Performance

Recent studies highlight challenges in antibody specificity. For example:

• A 2023 analysis of 614 commercial antibodies found significant variability in performance across applications (WB, IP, IF) .

• Protocols like standardized antigen retrieval (e.g., citrate buffer pH 6 for IHC) improve reproducibility .

Comparative Analysis of Antibody Applications

Research Implications

• RAB7: Critical for studying autophagy and endosomal trafficking .

• RPL7: Useful in translational regulation and cancer biology (e.g., lung carcinoma studies) .

Notes

• The term "RBL7" may refer to a typographical error. No antibody matching this designation was identified in the provided sources.

• For rigorous experimental design, always include controls (e.g., KO samples, isotype controls) and validate antibodies across multiple platforms .