RANBP1A Antibody

Overview of RANBP1 and Antibody Relevance

RANBP1 is a critical regulator of the RAN GTPase cycle, essential for nucleocytoplasmic transport, mitotic spindle assembly, and cell cycle progression . Antibodies targeting RANBP1 are vital tools for studying its role in cancer biology, intracellular transport, and epigenetic regulation.

Key Antibody Characteristics

Commercial RANBP1 antibodies are validated for applications including Western blot (WB), immunocytochemistry (ICC), and immunofluorescence (IF). Below is a comparative analysis of prominent RANBP1 antibodies:

Molecular Mechanisms

• Nuclear Transport: RANBP1 antibodies help elucidate its role in dissociating RAN from importin-β and KPNA2 complexes, ensuring cargo release .

• Mitotic Regulation: Antibody-based studies confirm RANBP1's necessity for spindle assembly and mitotic progression .

Epigenetic and Diagnostic Utility

• RANBP1 antibodies aid in detecting overexpression in tumors (AUC = 0.84 in HCC diagnosis) .

• Protein-level validation via immunohistochemistry shows upregulated RANBP1 in HCC vs. normal tissues .

Limitations and Considerations

• Specificity: Some antibodies (e.g., ab2937) show a 27 kDa band vs. the predicted 23 kDa, possibly due to post-translational modifications .

• Species Reactivity: Most antibodies are human-specific; cross-reactivity with murine models requires validation .