rbx-1 Antibody
Description
Overview of RBX-1 Antibodies
RBX-1 antibodies are monoclonal or polyclonal reagents designed to detect and study the RBX1 protein in various experimental settings. Key commercial products include:
| Antibody Name | Clone/Product ID | Applications | Reactivity | Vendor |
|---|---|---|---|---|
| Anti-RBX1 [EPR6850(B)] | ab133565 | WB, IHC-P, IP, ICC/IF, Flow Cytometry | Human, Mouse, Rat | Abcam |
| RBX1 Antibody | #4397 | WB | Human, Mouse, Rat, Monkey | Cell Signaling Technology |
| RBX1 (D3J5I) Rabbit mAb | #11922 | WB, IP, IHC | Human, Mouse, Rat, Monkey | Cell Signaling Technology |
These antibodies target endogenous RBX1, which migrates at ~13 kDa in Western blot (WB) analyses .
Western Blotting
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Band Specificity: Detects endogenous RBX1 at 13 kDa in human, mouse, and rat lysates .
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Validation: ab133565 shows reactivity in heart lysates across species (human, mouse, rat) , while #11922 confirms RBX1 expression in cancer cell lines (e.g., HeLa) .
Immunohistochemistry (IHC)
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Tissue Staining: RBX1 overexpression correlates with poor prognosis in endometrial carcinoma (EC) and triple-negative breast cancer (TNBC) . Antibodies like #11922 (1:1,200–1:4,800 dilution) enable RBX1 detection in formalin-fixed paraffin-embedded tissues .
Functional Studies
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Cancer Research: High RBX1 expression is linked to advanced tumor stages, lymph node metastasis, and chemoresistance in EC . Silencing RBX1 inhibits TNBC cell migration and epithelial-mesenchymal transition (EMT) .
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DNA Damage Response: RBX1 depletion causes CDT1/ORC1 accumulation, triggering DNA double-strand breaks and aneuploidy . Antibodies like ab133565 are used to study RBX1’s role in restraining homologous recombination repair via EXO1 degradation .
Role in Cancer Progression
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Prognostic Marker: RBX1 overexpression predicts poor survival in EC (P=0.004) and TNBC (P<0.001) .
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Mechanistic Insights:
DNA Repair Regulation
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G1-Phase Restriction: RBX1 degrades EXO1 via SCF ligase activity, limiting homologous recombination (HR) in G1-phase cells .
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Genomic Stability: RBX1 deficiency induces DNA replication licensing errors, leading to chromosomal instability .
Validation Data
| Antibody | Key Validation |
|---|---|
| ab133565 | Confirmed in Jurkat (flow cytometry) and HeLa cells (ICC) . |
| #11922 | Validated in human breast cancer tissues (IHC) and co-immunoprecipitation assays . |
Cross-Reactivity
Clinical Relevance
RBX1 is a promising therapeutic target due to its dual role in oncogenesis and DNA repair. Antibodies enable:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
