Customize rec-8 Antibody

Description

Antibody Characteristics

REC8 antibody is available in polyclonal and monoclonal formats, with key properties including:

Property	Details	Source Reference
Host Species	Rabbit (polyclonal and monoclonal)
Immunogen	- Polyclonal: REC8 fusion protein (C-terminal residues) or synthetic peptides (AA 22-120) - Monoclonal: Recombinant protein epitope
Reactivity	Human, Mouse, Rat (validated); Dog, Pig, Horse (predicted)
Molecular Weight	- Calculated: 63 kDa - Observed: 85–95 kDa (due to post-translational modifications)
Applications	Western Blot (WB), Immunofluorescence (IF), IHC, ELISA, Flow Cytometry (FACS)

Key Research Applications

REC8 antibodies are widely used to study chromosomal dynamics and antiviral immune responses:

Meiotic Chromosome Regulation

Role in Cohesion: REC8 maintains sister chromatid cohesion during meiosis I/II. Cleavage by separin enables chromosome separation .
Tissue Expression: Highly expressed in human testis, thymus, and B-cell lines .

Innate Immune Modulation

REC8 stabilizes antiviral signaling proteins MAVS and STING by:

Inhibiting Ubiquitination: Blocks RNF5-mediated K48-linked ubiquitination, preventing degradation of MAVS/STING .
Promoting TBK1 Recruitment: Enhances IRF3 and NF-κB activation, boosting IFN-β and ISG production during RNA/DNA virus infection .
SUMOylation Dependency: Viral infection triggers SUMOylation at Lys30/530, enabling REC8’s cytoplasmic translocation .

Recommended Dilutions

Application	Dilution Range	Buffer Conditions
Western Blot	1:500 – 1:20,000	PBS with 0.02% sodium azide
Immunofluorescence	1:50 – 1:200	Tris/EDTA pH 9.0 (IHC-P)
ELISA	1:500 – 1:1,000	1% BSA in TBS

Note: Optimal dilutions vary by sample type; validation using positive controls (e.g., human testis lysate) is advised .

Research Findings Table

Study Focus	Key Outcome	Method Used
Antiviral Signaling	REC8 knockdown reduces IFN-β/ISG expression during VSV, NDV, and HSV infection	siRNA, qRT-PCR, Luciferase
Protein Interaction	REC8 colocalizes with MAVS (Pearson’s R = 0.89) in VSV-infected cells	Co-IP, Immunofluorescence
Post-Translational Modification	SUMOylation at Lys30/530 essential for cytoplasmic translocation	Mutagenesis, WB

Limitations and Considerations

Species Specificity: Mouse/Rat reactivity may require validation for novel models .
Batch Variability: Polyclonal antibodies may exhibit lot-to-lot variability .
Storage: Stable at -20°C for 1 year; avoid freeze-thaw cycles .

Product Specs

Buffer

**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

rec-8 antibody; W02A2.6 antibody; Meiotic recombination protein rec-8 antibody; Cohesin rec-8 antibody

Target Names

rec-8

Uniprot No.

Q9XUB3

Target Background

Function

REC-8 antibody plays a crucial role in meiotic chromosome cohesion and segregation. It is essential for sister chromatid co-orientation and cohesion until meiosis II. Additionally, it may be required for the repair of programmed meiotic double-stranded breaks. During spermatogenesis, REC-8 is likely involved in spermatocyte centriole cohesion, preventing their separation during the second meiotic division.

Database Links

KEGG: cel:CELE_W02A2.6

STRING: 6239.W02A2.6a

UniGene: Cel.12445

Protein Families

Rad21 family

Subcellular Location

Chromosome. Nucleus. Cytoplasm, cytoskeleton, spindle pole.

Tissue Specificity

Expressed in premeiotic and meiotic gonadal cells. Expressed in spermatocytes.

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rec-8 Antibody