At3g10140 Antibody
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Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
At3g10140 antibody; T22K18.3 antibody; DNA repair protein recA homolog 2 antibody; mitochondrial antibody; Recombinase A homolog 2 antibody
Target Names
At3g10140
Uniprot No.
Target Background
Function
At3g10140 antibody targets a protein that plays a critical role in recombination ability and DNA strand transfer activity.
Gene References Into Functions
- RECA2 and RECA3 exhibit similar expression patterns and functionally complement bacterial RecA. Mutations in recA2 and recA3 genes result in rearranged mitochondrial DNA. Furthermore, DNA break repair is compromised in the absence of RECA3. PMID: 22415515
Database Links
Protein Families
RecA family
Subcellular Location
Mitochondrion.
Q&A
Advanced Research Questions
How to resolve contradictory localization data between studies?
Conduct a methodological comparison table:
| Parameter | Study A (2022) | Study B (2023) | Recommended Resolution |
|---|---|---|---|
| Fixation method | 4% PFA 30 min | Methanol:Acetone 1:1 | Test both protocols with same material |
| Antibody dilution | 1:500 | 1:1000 | Titration curve (1:100-1:2000) |
| Detection system | Alkaline phos. | Fluorescent | Parallel staining with both methods |
How to address batch-to-batch variability in long-term studies?
Establish a validation matrix:
| Batch | Lot # | EC50 (nM) | Cross-reactivity (%) | Reference Standard Δ |
|---|---|---|---|---|
| 1 | X234 | 1.8 ± 0.3 | 2.1 | +0.2σ |
| 2 | Y567 | 2.1 ± 0.4 | 3.8 | -0.5σ |
| 3 | Z890 | 1.9 ± 0.2 | 1.9 | +0.1σ |
Normalize data using Z-score transformation when |Δ| >0.5σ
Methodological Recommendations
6. Optimal tissue processing for phloem-specific protein detection:
-
Fixation: 3% formaldehyde + 0.01% glutaraldehyde (4°C, 2 hr)
-
Sectioning: 40 μm vibratome sections with 0.5% periodic acid pretreatment
-
Signal amplification: Tyramide-based system with 12 min substrate incubation
7. Strategies for epitope mapping in structure-function studies:
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