Recombinant 60S ribosomal protein L12 (rpl-12)

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Cat. No.
BT39920
Source
Yeast
Synonyms
rpl-12; CBG01806; 60S ribosomal protein L12
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39920
Source
E.coli
Synonyms
rpl-12; CBG01806; 60S ribosomal protein L12
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39920
Source
E.coli
Synonyms
rpl-12; CBG01806; 60S ribosomal protein L12
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39920
Source
Baculovirus
Synonyms
rpl-12; CBG01806; 60S ribosomal protein L12
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39920
Source
Mammalian cell
Synonyms
rpl-12; CBG01806; 60S ribosomal protein L12
Purity
>85% (SDS-PAGE)
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Description

Recombinant Production

Recombinant RPL12 is produced via heterologous expression systems, including:

SourceExpression SystemPurityMolecular Weight
Boster Bio HEK293T cells>80%17.6 kDa
Abcam E. coli>95%20.2 kDa
Prospec Bio E. coli>95%20.2 kDa

Biological Function

  • Ribosome assembly: Binds directly to 26S rRNA and facilitates the maturation of the 60S subunit in the nucleolus .

  • Unique nuclear import: Utilizes importin 11, distinct from the general ribosomal protein import pathway, to enter the nucleus .

  • Tick physiology: Expressed predominantly in the ovaries of Haemaphysalis longicornis, with roles in feeding and egg development .

Research Applications

  • Vaccine development: Recombinant RPL12 (rRpL12) shows promise as a tick vaccine candidate, reducing egg weight and ovary development by 17.22% and 62.45%, respectively .

  • Protein synthesis regulation: Ubiquitination of RPL12 modulates ribosome function during cellular stress .

  • Structural studies: X-ray crystallography and SDS-PAGE analyses reveal structural homology with bacterial homologs (e.g., E. coli L12) .

Amino Acid Sequence Conservation

SpeciesIdentity (%)Key Features
H. longicornis98.76%Phosphorylation sites at Ser38 and Ser124
Drosophila82%Conserved uL11 family motifs
Dermacentor~90%High sequence similarity in tick genera

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. Request dry ice shipment in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us and we will prioritize its development.
Synonyms
rpl-12; CBG01806; 60S ribosomal protein L12
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-165
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Caenorhabditis briggsae
Target Names
rpl-12
Target Protein Sequence
MPPKFDPTEI KIVYLRCVGG EVGATSALAP KVGPLGLSPK KIGEDIAKAT QDWKGLKVTC KLTIQNRVAK IDVVPSAASL IVKELKEPPR DRKKVKNVKH NGDLTVDTII KIARIMRPRS MAKKLEGTVK EILGTAQSVG CTIDGQHPHD IIESIANGEI EIPAQ
Uniprot No.
P61865

Target Background

Function
Binds directly to 26S ribosomal RNA.
Database Links

KEGG: cbr:CBG01806

STRING: 6238.CBG01806

Protein Families
Universal ribosomal protein uL11 family

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