Recombinant Acanthamoeba castellanii NADH-ubiquinone oxidoreductase chain 3 (ND3)
Description
Structure and Function
ND3 (UniProt ID: Q37382) is a 118-amino acid protein (1–118 aa) with a molecular weight of ~13 kDa. It is fused to an N-terminal His tag for purification and contains a hydrophobic transmembrane domain, consistent with its role as a core subunit in complex I .
Key Features
Complex I, of which ND3 is a subunit, is a ~940 kDa multi-protein assembly responsible for transferring electrons from NADH to ubiquinone while pumping protons across the inner mitochondrial membrane . In Acanthamoeba, ND3 is part of a larger complex that includes γ-type carbonic anhydrases (γCA), suggesting a conserved evolutionary role in mitochondrial function .
Production and Biochemical Properties
ND3 is expressed in E. coli and purified via nickel affinity chromatography due to its His tag. Post-production, it is lyophilized and stored at -20°C/-80°C to maintain stability .
Key Findings
Product Specs
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The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
