Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L448 (MIMI_L448)
Description
Overview
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L448 (MIMI_L448) is a protein found in the Acanthamoeba polyphaga mimivirus (APMV), a giant virus that infects amoeba . APMV possesses a large genome encoding for numerous proteins, some of which have unknown functions . Research suggests that certain uncharacterized proteins within Mimivirus are essential for the generation of infectious viral particles .
Discovery and Background
The Acanthamoeba polyphaga mimivirus (APMV) was initially discovered in 2003 . The discovery of APMV revealed the presence of various proteins and RNA within the virion . The roles of these proteins and RNA are suggested to be linked to the early stages of infection, but have not been thoroughly investigated .
Function and Significance
MIMI_L448 is one of the many uncharacterized proteins encoded by the mimivirus genome . Studies using microinjection of mimivirus DNA into Acanthamoeba castellanii have identified several uncharacterized proteins, including L442, L724, L829, and R387, that are necessary for DNA-mediated APMV generation . Protein analysis using SDS-PAGE revealed five putative protein bands, with band no. 3 containing the cleaved sequence of the uncharacterized protein L442, which has a size between 43 and 55,223 kDa . Other mimivirus proteins identified include GMC-type oxidoreductase R135, uncharacterized protein R387, and uncharacterized proteins L724 and L829 .
Experimental Analysis
To investigate the necessity of proteins associated with APMV DNA for viral production, researchers have used proteinase K to eliminate residual proteins from extracted DNA . Microinjection experiments with and without proteinase K pre-treatment showed that viral production occurred in experiments without proteinase K treatment, whereas experiments with proteinase K pre-treated APMV DNA did not lead to amoeba monolayer lysis associated with APMV particle production .
Tables
| Protein Name | Molecular Weight (kDa) |
|---|---|
| Uncharacterized protein L442 | 43-55,223 |
| GMC-type oxidoreductase R135 | 76,947 |
| Uncharacterized protein R387 | 30,067 |
| Uncharacterized protein L724 | 24,033 |
| Uncharacterized protein L829 | 49,226 |
| Experiment | Viral Production |
|---|---|
| Microinjection without Proteinase K treatment | Yes |
| Microinjection with Proteinase K pre-treatment | No |
It is important that tables are constructed in a way that they are easily understood on their own, without needing to refer to the main text of the article .
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Target Background
KEGG: vg:9925072
