Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L885 (MIMI_L885)
Description
Definition and Basic Properties
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L885 (MIMI_L885) is a full-length recombinant protein derived from the Acanthamoeba polyphaga mimivirus (APMV), a member of the Mimiviridae family. The protein is expressed in E. coli with an N-terminal His-tag for purification and contains 144 amino acids (UniProt ID: Q5UQY5) .
| Property | Detail |
|---|---|
| Source Organism | Acanthamoeba polyphaga mimivirus (APMV) |
| Protein Length | 1–144 amino acids (full-length) |
| Tag | N-terminal His-tag |
| Purity | >85% (SDS-PAGE) |
| Storage | Lyophilized powder stored at -20°C/-80°C; avoid repeated freeze-thaw cycles |
| Reconstitution | Deionized sterile water (0.1–1.0 mg/mL) with 5–50% glycerol |
Amino Acid Sequence
The sequence begins with MDKLVPDVFK... and terminates with ...YILSEY, featuring a mix of hydrophobic and hydrophilic regions. While no specific structural motifs are annotated, bioinformatics tools like Phyre2 may predict secondary/tertiary structures (e.g., α-helices or β-sheets) .
| Sequence Segment | Amino Acid Stretch |
|---|---|
| N-terminal | MDKLVPDVFK NKISDIIIED DFDKENLVQE TIACIEFDNF ECKFECYKFQ SSKSDSPESD ISHNINIYTP |
| C-terminal | ...YILSEY (partial) |
Protein Expression and Purification
MIMI_L885 is expressed in E. coli using a tetracycline-inducible system and purified via affinity chromatography (His-tag). Purity is confirmed by SDS-PAGE, with no contaminants detected .
Hypothetical Roles
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Viral Replication: Analogous to other APMV proteins (e.g., L442, L724), MIMI_L885 may assist in viral factory formation or DNA packaging .
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Host Interaction: Potential involvement in modulating host cell membranes or immune evasion, common among NCLDVs .
Experimental Approaches
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Structural Studies: Crystallization and X-ray diffraction (as done for NDK in ) could elucidate its 3D structure.
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Functional Assays: Knockout or overexpression in A. castellanii to assess effects on viral production or host cell lysis .
Comparative Analysis with Other Mimivirus Proteins
| Protein | UniProt ID | Length (aa) | Tag | Purity | Source |
|---|---|---|---|---|---|
| L885 (MIMI_L885) | Q5UQY5 | 144 | His | >85% | |
| R809 (MIMI_R809) | Q5UQI2 | 229 | His | >90% | |
| R203 (MIMI_R203) | Q5UQ26 | 337 | His | >85% |
Key Observations:
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Expression Systems: All listed proteins are His-tagged and expressed in E. coli.
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Purity Standards: L885 and R203 meet >85% purity, while R809 exceeds 90% .
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Functional Diversity: R809 (uncharacterized) and R203 (uncharacterized) highlight the prevalence of understudied proteins in APMV .
Technical Limitations
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Lack of Functional Data: No enzymatic activity or binding partners identified for L885.
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Structural Complexity: APMV’s large genome (~1.2 Mbp) complicates targeted studies .
Prioritized Research Areas
Product Specs
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The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
