Recombinant Acidiphilium cryptum ATP synthase subunit beta (atpD)

Shipped with Ice Packs
In Stock
Cat. No.
BT43279
Source
Yeast
Synonyms
atpD; Acry_1681ATP synthase subunit beta; EC 7.1.2.2; ATP synthase F1 sector subunit beta; F-ATPase subunit beta
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43279
Source
E.coli
Synonyms
atpD; Acry_1681ATP synthase subunit beta; EC 7.1.2.2; ATP synthase F1 sector subunit beta; F-ATPase subunit beta
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43279
Source
E.coli
Synonyms
atpD; Acry_1681ATP synthase subunit beta; EC 7.1.2.2; ATP synthase F1 sector subunit beta; F-ATPase subunit beta
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43279
Source
Baculovirus
Synonyms
atpD; Acry_1681ATP synthase subunit beta; EC 7.1.2.2; ATP synthase F1 sector subunit beta; F-ATPase subunit beta
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43279
Source
Mammalian cell
Synonyms
atpD; Acry_1681ATP synthase subunit beta; EC 7.1.2.2; ATP synthase F1 sector subunit beta; F-ATPase subunit beta
Purity
>85% (SDS-PAGE)
Quick Inquiry

Description

Introduction to ATP Synthase Subunit Beta (atpD)

The beta subunit of ATP synthase (encoded by atpD) is essential for catalytic ATP synthesis, utilizing proton gradients across membranes to phosphorylate ADP into ATP . In Acidiphilium cryptum, a bacterium thriving in acidic environments, this subunit enables energy production under extreme pH conditions. Recombinant versions allow targeted studies of ATP synthase mechanics and microbial adaptations .

Biochemical Characteristics of Recombinant A. cryptum atpD

Key properties of the recombinant protein include:

ParameterDetails
UniProt IDA5FZ54
SourceAcidiphilium cryptum (strain JF-5)
Expression SystemEscherichia coli
Purity>85% (verified via SDS-PAGE)
Storage-20°C (short-term); -80°C for long-term stability
Sequence Length474 amino acids (partial sequence provided below)

Data derived from product specifications and peer-reviewed studies .

Functional and Evolutionary Significance

  • Catalytic Role: The beta subunit facilitates ATP synthesis by undergoing conformational changes during rotational catalysis, driven by proton motive force .

  • Evolutionary Conservation: Comparative genomic analyses reveal atpD as one of the least variable housekeeping genes in bacteria, underscoring its essential role in energy metabolism .

  • Research Applications: Recombinant atpD enables studies on ATP synthase inhibition, microbial energetics in extreme environments, and enzyme engineering for biotechnological uses .

Research Limitations and Future Directions

Current data gaps include:

  1. Structural Dynamics: High-resolution cryo-EM or crystallographic data for A. cryptum atpD remain unpublished.

  2. Inhibitor Binding Studies: No reported investigations into small-molecule interactions with the recombinant protein.

  3. Environmental Adaptations: Mechanistic links between atpD structure and acidophilic metabolism require further exploration .

Product Specs

Form
Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery time varies depending on the purchase method and location. Consult your local distributors for specific delivery times. All proteins are shipped with standard blue ice packs. Requesting dry ice shipment requires prior communication and incurs extra fees.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a 6-month shelf life at -20°C/-80°C. The lyophilized form has a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receiving. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be decided during the manufacturing process. If you require a specific tag, please inform us, and we will prioritize developing it.
Synonyms
atpD; Acry_1681ATP synthase subunit beta; EC 7.1.2.2; ATP synthase F1 sector subunit beta; F-ATPase subunit beta
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-476
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Acidiphilium cryptum (strain JF-5)
Target Names
atpD
Target Protein Sequence
MAKNVTGRIT QIMGPVVDVQ FEGELPYILN ALETRVGDRR LVLEVAQEIG ERTVRCIAMD STDGLARGDE VRDTGEAIAV PVGPETLGRI LNVIGEPIDE RGPIPTTRTA PIHRQAPSFD EQATSAEILV TGIKVVDLLA PYLKGGKIGL FGGAGVGKTV LIQELINNIA KGHGGVSVFA GVGERTREGN DLYHEMIDAG VIKLGEGTTE GSKVALVYGQ MNEPPGARMR VGLSGLTMAE YFRDEEGQDV LFFVDNIFRF TQAGAEVSAL LGRIPSAVGY QPTLATDMGA LQERITSTKK GSITSVQAIY VPADDLTDPA PATSFSHLDA TTTLNRAIAE KGIYPAVDPL DSTSRALDPR IVGEEHYNVA REVQRILQSY KSLQDIIAIL GMDELSEDDK LTVARARKIE RFLSQPFHVA EVFTGSPGIF VPVEDTVRSF KAICAGEYDH LPEAAFYMVG TIEDAVKKAE SLNATA
Uniprot No.
A5FZ54

Target Background

Function
Generates ATP from ADP using a proton gradient across the membrane. The catalytic sites are mainly located in the beta subunits.
Database Links

KEGG: acr:Acry_1681

STRING: 349163.Acry_1681

Protein Families
ATPase alpha/beta chains family
Subcellular Location
Cell inner membrane; Peripheral membrane protein.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2025 TheBiotek. All Rights Reserved.