Recombinant Acidobacterium capsulatum Protein CrcB homolog (crcB)
Description
Introduction to Recombinant Acidobacterium capsulatum Protein CrcB Homolog (crcB)
The Recombinant Acidobacterium capsulatum Protein CrcB homolog (crcB) is a recombinant protein derived from the bacterium Acidobacterium capsulatum. This protein is often expressed in Escherichia coli (E. coli) and is typically fused with an N-terminal His tag to facilitate purification and detection. The CrcB protein is of interest due to its potential role in fluoride ion transport, which helps reduce fluoride toxicity within cells .
2.2. Amino Acid Sequence
The amino acid sequence of the Recombinant Acidobacterium capsulatum Protein CrcB homolog is as follows:
3.1. Role in Fluoride Transport
CrcB proteins are predicted to function as fluoride transporters, helping to reduce cellular fluoride concentrations and mitigate its toxicity. Studies in other organisms have shown that CrcB proteins are crucial for survival in environments with high fluoride levels .
3.2. Expression and Purification
The recombinant protein is expressed in E. coli, which provides a robust system for large-scale production. The His tag facilitates purification using affinity chromatography, ensuring high purity of the final product .
4.1. Salmonella Dublin CrcB Homolog
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Species: Salmonella dublin
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Source: Expressed in E. coli
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Tag: N-terminal His tag
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Protein Length: Full-length, 127 amino acids
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Form: Lyophilized powder
4.2. Comparison Table
| Feature | Acidobacterium capsulatum CrcB | Salmonella dublin CrcB |
|---|---|---|
| Species | Acidobacterium capsulatum | Salmonella dublin |
| Source | E. coli | E. coli |
| Tag | N-terminal His tag | N-terminal His tag |
| Protein Length | 125 amino acids | 127 amino acids |
| Form | Lyophilized powder | Lyophilized powder |
| Purity | >90% | >90% |
| Storage | -20°C/-80°C | -20°C/-80°C |
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
The tag type is determined during the production process. If you require a specific tag type, please inform us; we will prioritize its development.
Target Background
KEGG: aca:ACP_3316
STRING: 240015.ACP_3316
