Recombinant Acidovorax citrulli ATP synthase subunit a (atpB)
Description
Recombinant Production of ATP Synthase Subunits
While recombinant AtpB from A. citrulli is not explicitly reported, methodologies for homologous subunits in related species provide a blueprint:
Table 1: Recombinant ATP Synthase Subunits in Acidovorax spp.
| Subunit | Species | Host System | Tag | Purity | Application | Source |
|---|---|---|---|---|---|---|
| AtpE | A. citrulli | E. coli | His | >90% | Structural studies | |
| AtpB | A. ebreus | E. coli | His | >85% | Functional assays | |
| AtpB | Unspecified | E. coli | His | N/A | Enzyme kinetics |
Production Workflow:
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Gene Cloning: Amplify atpB from A. citrulli genomic DNA (e.g., strain M6, GenBank CP000512.1) .
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Vector Construction: Use plasmids like pET or pGEX for expression in E. coli .
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Purification: Affinity chromatography (e.g., Ni-NTA for His-tagged proteins) followed by size-exclusion chromatography .
Research Implications and Challenges
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Functional Redundancy: ATP synthase subunits in A. citrulli may exhibit functional overlap, as seen in other bacteria .
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Pathogenicity Link: ATP synthase is vital for bacterial survival under stress (e.g., oxidative or thermal), which influences virulence .
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Knowledge Gaps: No direct studies on A. citrulli AtpB exist; structural predictions rely on homologs like A. ebreus AtpB (UniProt B9MB97) .
Future Directions
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Structural Studies: Cryo-EM or X-ray crystallography to resolve AtpB’s proton channel mechanism.
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Gene Knockout Models: Assess atpB deletion effects on A. citrulli motility and biofilm formation, as done for ClpA .
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Plant Interaction Assays: Test recombinant AtpB’s role in suppressing host immunity, akin to effector proteins like AopV .
Product Specs
Please note: We will prioritize shipping the format currently in stock. However, if you have a specific format preference, please indicate it when placing your order. We will fulfill your request if possible.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance as additional charges will apply.
Generally, liquid form has a shelf life of 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: aav:Aave_0366
STRING: 397945.Aave_0366
