Recombinant Actinobacillus pleuropneumoniae serotype 3 Large-conductance mechanosensitive channel (mscL)
Description
General Information
Actinobacillus pleuropneumoniae is a Gram-negative bacterium that causes porcine contagious pleuropneumonia (PCP), a severe respiratory disease in pigs, leading to significant economic losses in the swine industry . A. pleuropneumoniae has various serotypes, with serotype 3 being a low-virulence serotype, although it can still cause disease .
Mechanosensitive channels (MSCs) are membrane proteins that respond to mechanical stimuli, such as changes in osmotic pressure, by opening a channel that allows ions and other small molecules to pass through the cell membrane . Large-conductance mechanosensitive channels (MscL) are a specific type of MSC that allows for the rapid efflux of solutes to alleviate osmotic stress . MscL plays a vital role in bacterial resistance to external environmental changes, ensuring normal bacterial growth .
Function and Significance of MscL in A. pleuropneumoniae
MscL in A. pleuropneumoniae is crucial for several functions :
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Osmotic Adaptation: MscL helps the bacterium adapt to changes in environmental osmolarity by regulating cell length, thus preventing drastic rises or falls in osmotic pressure .
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Antibiotic Resistance: MscL influences the bacterium's sensitivity to multiple antibiotics. Deletion of the mscL gene can decrease the sensitivity of A. pleuropneumoniae to antibiotics such as chloramphenicol, erythromycin, and penicillin .
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Biofilm Formation: MscL plays a role in biofilm formation, which is a critical factor in bacterial survival and virulence .
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Virulence: MscL and MscS do not affect the virulence of A. pleuropneumoniae .
MscL Structure and Homology
The MscL sequence of A. pleuropneumoniae shares significant homology with MscL from other bacterial species, such as E. coli . Amino acid sequence identity between A. pleuropneumoniae and E. coli MscL is approximately 76% . Conserved motifs, such as transmembrane domains (TM1 and TM2), are present in the MscL sequence of A. pleuropneumoniae, with high homology to E. coli .
Impact of MscL Deletion on Antibiotic Sensitivity
Deletion of the mscL gene affects the sensitivity of A. pleuropneumoniae to various antibiotics :
Recombinant Production and Applications
Recombinant MscL protein can be produced using various expression systems. It is used in research for studying the function and characteristics of the channel . Recombinant MscL is available for purchase for research purposes .
Table of Internalization Percentage of A. pleuropneumoniae Strains
The internalization capacity of different A. pleuropneumoniae strains in swine aortic endothelial cells is shown in the table below :
| Strain | Internalization Percentage |
|---|---|
| S4074 | 1.3% |
| JL03 | 4.6% |
| L20 | 1.75% |
| AP76 | 1.6% |
Product Specs
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Target Background
KEGG: apj:APJL_1625
