Recombinant Actinobacillus succinogenes ATP synthase subunit alpha (atpA), partial

Shipped with Ice Packs
In Stock
Cat. No.
BT43317
Source
Yeast
Synonyms
atpA; Asuc_0328ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43317
Source
E.coli
Synonyms
atpA; Asuc_0328ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43317
Source
E.coli
Synonyms
atpA; Asuc_0328ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43317
Source
Baculovirus
Synonyms
atpA; Asuc_0328ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43317
Source
Mammalian cell
Synonyms
atpA; Asuc_0328ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Purity
>85% (SDS-PAGE)
Quick Inquiry

Description

Definition and Biological Role

ATP synthase subunit alpha (atpA) is a critical component of the ATP synthase complex, which catalyzes ATP production via oxidative phosphorylation. In A. succinogenes, this enzyme plays a role in energy metabolism during anaerobic fermentation, particularly in pathways linked to succinic acid (SA) biosynthesis .

Recombinant Expression and Applications

While no direct studies on recombinant A. succinogenes AtpA were found, genetic engineering in this organism is well-documented. For example:

  • Plasmid Systems: A. succinogenes has been engineered using shuttle vectors (e.g., pLGZ901/pLGZ920) for gene overexpression or knockout .

  • Metabolic Flux Analysis: Genome-scale models (e.g., iBP722) predict ATP synthase’s role in redirecting carbon flux toward SA production under anaerobic conditions .

Key Research Gaps and Challenges

  • Sequence Data: The atpA gene in A. succinogenes has not been explicitly annotated in the provided sources.

  • Functional Studies: No experimental data on recombinant AtpA purification, kinetics, or structural analysis were identified.

Comparative Insights from Related Organisms

A recombinant ATP synthase subunit alpha from Xanthomonas oryzae (UniProt: Q5H4Y6) was documented , highlighting methodological frameworks that could be adapted for A. succinogenes:

ParameterDetails
Product CodeCSB-EP688963XBB-B
Expression HostEscherichia coli
Purity>85% (SDS-PAGE)
Storage-20°C/-80°C (lyophilized form stable for 12 months)

Future Directions

  • Gene Annotation: Prioritize functional annotation of atpA in A. succinogenes using genomic databases.

  • Heterologous Expression: Leverage established vectors (e.g., pLGZ920) to express and characterize AtpA .

  • Metabolic Impact: Investigate ATP synthase’s role in SA yield optimization using constraint-based metabolic models .

Product Specs

Form
Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering.
Lead Time
Delivery time varies by purchase method and location. Consult local distributors for specific delivery times. All proteins are shipped with blue ice packs by default. Contact us in advance for dry ice shipping (extra fees apply).
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer ingredients, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you have a specific tag type requirement, please inform us.
Synonyms
atpA; Asuc_0328ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Actinobacillus succinogenes (strain ATCC 55618 / 130Z)
Target Names
atpA
Uniprot No.
A6VL59

Target Background

Function
Produces ATP from ADP using a proton gradient across the membrane. The alpha chain is a regulatory subunit.
Database Links

KEGG: asu:Asuc_0328

STRING: 339671.Asuc_0328

Protein Families
ATPase alpha/beta chains family
Subcellular Location
Cell inner membrane; Peripheral membrane protein.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2025 TheBiotek. All Rights Reserved.