Recombinant Actinobacillus succinogenes ATP synthase subunit c (atpE)
Shipped with Ice Packs 
In Stock 
Description
Production and Purification
The recombinant atpE is synthesized via heterologous expression in E. coli. Key steps include:
| Step | Details |
|---|---|
| Expression Host | E. coli |
| Induction Conditions | Standard protocols for recombinant protein expression (not specified) |
| Purification Method | Affinity chromatography (His-tag) |
| Reconstitution | Deionized sterile water (0.1–1.0 mg/mL); glycerol added for stability |
The protein is stored at -20°C/-80°C to maintain activity, with repeated freeze-thaw cycles discouraged .
Suppliers and Availability
The recombinant atpE is commercially available through specialized biotechnology vendors, such as CUSABIO and Creative Biomart.
| Supplier | Product Code | Key Features |
|---|---|---|
| CUSABIO | CB615628327 | Lyophilized powder; >90% purity |
| Creative Biomart | RFL35319AF | Full-length His-tagged protein; E. coli-expressed |
Research Gaps and Future Directions
While structural data for atpE is available, functional studies in Actinobacillus succinogenes remain sparse. Key areas for investigation include:
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.
Lead Time
Delivery times vary depending on the purchase method and location. Please contact your local distributor for precise delivery estimates.
Note: All protein shipments default to blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
Note: All protein shipments default to blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and serves as a guideline.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during manufacturing.
If you require a specific tag, please inform us, and we will prioritize its inclusion in the production process.
If you require a specific tag, please inform us, and we will prioritize its inclusion in the production process.
Synonyms
atpE; Asuc_0331; ATP synthase subunit c; ATP synthase F(0 sector subunit c; F-type ATPase subunit c; F-ATPase subunit c; Lipid-binding protein
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-84
Protein Length
full length protein
Species
Actinobacillus succinogenes (strain ATCC 55618 / 130Z)
Target Names
atpE
Target Protein Sequence
MESVITATIIGASILLAFAALGTAIGFAILGGKFLESSARQPELASSLQTKMFIVAGLLD
AIAMIAVGISLLFIFANPFISLLQ
Uniprot No.
Target Background
Function
F(1)F(0) ATP synthase synthesizes ATP from ADP using a proton or sodium gradient. This enzyme comprises two domains: the extramembranous catalytic F(1) domain and the membrane-bound proton channel F(0) domain, connected by a central and peripheral stalk. ATP synthesis in the F(1) catalytic domain is coupled to proton translocation via a rotary mechanism involving the central stalk subunits. A key component of the F(0) channel, subunit c directly participates in transmembrane translocation. A homomeric c-ring, consisting of 10-14 subunits, forms the central stalk rotor element in conjunction with the F(1) delta and epsilon subunits.
Database Links
KEGG: asu:Asuc_0331
STRING: 339671.Asuc_0331
Protein Families
ATPase C chain family
Subcellular Location
Cell inner membrane; Multi-pass membrane protein.
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