Recombinant Adenosine monophosphate-protein transferase FICD homolog (ZK593.8)
Description
Introduction to Recombinant Adenosine Monophosphate-Protein Transferase FICD Homolog (ZK593.8)
Recombinant Adenosine monophosphate-protein transferase FICD homolog (ZK593.8) is a recombinant protein derived from the gene fic-1. This protein is part of a family of enzymes known as adenosine monophosphate-protein transferases, which are involved in various cellular processes, including protein modification and signaling pathways. The FICD homologs are particularly noted for their role in AMPylation, a post-translational modification that can affect protein function and stability.
Gene and Protein Information
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Gene Name: fic-1
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Other Names: Adenosine monophosphate-protein transferase FICD homolog
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Host/Reactivities: This recombinant protein can be expressed in various hosts, including E. coli, yeast, baculovirus, and mammalian cells .
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Purity: The purity of the recombinant protein is typically greater than or equal to 85%, as determined by SDS-PAGE .
Expression and Purification
The expression of recombinant Adenosine monophosphate-protein transferase FICD homolog (ZK593.8) involves the use of cell-free expression systems or traditional cell-based systems like E. coli or mammalian cells. The purification process often involves chromatography techniques to achieve high purity levels.
Biological Function
Adenosine monophosphate-protein transferases, including the FICD homologs, are known for their ability to transfer AMP from ATP to target proteins, a process known as AMPylation. This modification can alter protein activity, localization, or stability. The specific biological functions of the ZK593.8 homolog are not extensively detailed in available literature, but its role is likely related to cellular signaling and protein regulation.
Data Tables
| Protein Characteristics | Description |
|---|---|
| Gene Name | fic-1 |
| Host/Reactivities | E. coli, Yeast, Baculovirus, Mammalian Cells |
| Purity | ≥ 85% (SDS-PAGE) |
| Expression System | Cell-free or Cell-based |
References MyBioSource. Recombinant Adenosine monophosphate-protein transferase FICD homolog (ZK593.8). Yuan, Li et al. TRIM-21 promotes proteasomal degradation of CED-1. eLife 2022;11:e76436. DOI: https://doi.org/10.7554/eLife.76436 Adenosine Monophosphate-Activated Protein Kinase. PMC. 2017.
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested in advance. Additional fees apply for dry ice shipping.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
- A link between FIC-1-mediated AMPylation and susceptibility to *Pseudomonas aeruginosa* has been established, demonstrating a connection between AMPylation and innate immunity in *C. elegans*. PMID: 27138431
