Recombinant Aeromonas salmonicida NADH-quinone oxidoreductase subunit K (nuoK)
Description
Functional Role in Bacterial Metabolism
nuoK contributes to the NADH dehydrogenase complex (Complex I), which catalyzes electron transfer from NADH to quinone, coupled with proton translocation across the membrane . This activity is critical for generating the proton motive force (PMF) required for ATP synthesis . In A. salmonicida, disruption of this complex could impair energy metabolism, potentially reducing virulence .
Genomic Context and Pathogenicity
A. salmonicida, a Gram-negative pathogen causing furunculosis in salmonids, encodes nuoK within its respiratory gene cluster. Recent genomic analyses of re-emergent strains in Chile and Germany revealed conserved sequences in nuoK across isolates, suggesting its role in metabolic adaptation and pathogen persistence .
Key Genomic Insights:
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Conservation: nuoK exhibits <15 single-nucleotide polymorphisms (SNPs) compared to reference strains, indicating high stability .
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Resistome Link: NADH dehydrogenase subunits are co-expressed with antibiotic resistance genes (sul1, tetA) in aquaculture-associated strains .
4.1. Superoxide Production and Virulence
In related bacteria (Vibrio cholerae), the NADH-quinone oxidoreductase generates reactive oxygen species (ROS) via FAD-mediated electron leakage, which modulates virulence gene expression . Although direct evidence in A. salmonicida is lacking, structural homology suggests nuoK may similarly influence oxidative stress responses and host adaptation .
4.2. Vaccine Development Potential
Closed genomes of A. salmonicida strains (e.g., Chilean isolates) have enabled epitope mapping of respiratory proteins like nuoK for autogenous vaccine design . These vaccines could target conserved regions to mitigate furunculosis outbreaks in aquaculture .
Supplier Information
The protein is commercially available as a lyophilized powder with His-tag purification:
| Supplier | CUSABIO TECHNOLOGY LLC |
|---|---|
| Catalog Number | RFL25989AF |
| Host Species | Aeromonas salmonicida |
| Application Notes | Not for human consumption; research use only |
Future Directions
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them in your order notes, and we will fulfill your request.
Note: All protein shipments are standardly equipped with blue ice packs. If you require dry ice shipping, please inform us in advance as additional fees will apply.
Target Background
KEGG: asa:ASA_1727
STRING: 382245.ASA_1727
