Recombinant Alcanivorax borkumensis Putative methyl-accepting chemotaxis AlkN (ABO_0106)

Introduction to Recombinant Alcanivorax borkumensis Putative Methyl-Accepting Chemotaxis AlkN (ABO_0106)

Recombinant Alcanivorax borkumensis Putative Methyl-Accepting Chemotaxis AlkN (ABO_0106) is a recombinant protein derived from the bacterium Alcanivorax borkumensis, a species known for its ability to degrade hydrocarbons. This protein is involved in chemotaxis, which is the process by which bacteria move towards or away from chemical stimuli. The ABO_0106 protein is specifically a methyl-accepting chemotaxis protein (MCP), which plays a crucial role in detecting environmental signals and guiding the bacterium's movement.

Characteristics of Recombinant Alcanivorax borkumensis Putative Methyl-Accepting Chemotaxis AlkN (ABO_0106)

• Source: The recombinant protein is produced in Escherichia coli (E. coli), a common host for recombinant protein expression due to its well-understood genetics and ease of manipulation .

• Purity: The protein has a purity of greater than 85% as determined by SDS-PAGE, indicating a high level of purification .

• Protein Length: The protein is partial, suggesting that it may not contain the full-length sequence of the native protein .

• Storage Conditions: The shelf life of the liquid form is typically 6 months at -20°C or -80°C, while the lyophilized form can last up to 12 months under the same conditions. Repeated freezing and thawing should be avoided .

Function and Role in Chemotaxis

Methyl-accepting chemotaxis proteins (MCPs) like ABO_0106 are essential components of bacterial chemotaxis systems. They detect chemical signals in the environment and transmit these signals through a series of protein interactions to control flagellar rotation, thereby directing the bacterium's movement. In the context of Alcanivorax borkumensis, these proteins are likely involved in guiding the bacterium towards hydrocarbon substrates, facilitating its role in hydrocarbon degradation.

Research Findings and Applications

While specific research on the ABO_0106 protein itself is limited, studies on related chemotaxis systems in Alcanivorax species highlight the importance of chemotaxis in hydrocarbon degradation. For example, Alcanivorax dieselolei has been shown to possess a chemotaxis operon that includes genes for MCPs, which are crucial for detecting and responding to alkanes . This suggests that similar mechanisms might be at play in Alcanivorax borkumensis, with proteins like ABO_0106 playing key roles.

Data Table: Characteristics of Recombinant Alcanivorax borkumensis Putative Methyl-Accepting Chemotaxis AlkN (ABO_0106)

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