Recombinant Alpha-tubulin N-acetyltransferase (LinJ25.1190, LinJ_25_1190)
Description
Molecular Identity and Production
Key identifiers:
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Gene: LINJ_25_1190 (Leishmania infantum genome annotation)
Expression systems:
| Expression Host | Purity | Tagging | Molecular Weight |
|---|---|---|---|
| E. coli | >85% | Untagged | ~27 kDa |
| Yeast | >85% | Variable | Full-length |
| Baculovirus | >85% | Variable | Full-length |
The protein is lyophilized for stability and reconstituted with glycerol for long-term storage at -20°C to -80°C .
Catalytic Domain
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Comprises residues 1–246, retaining full enzymatic activity .
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Shares homology with human αTAT1, including a conserved acetyl-CoA binding pocket and substrate-binding groove . Critical residues (e.g., R69, H75, K102 in humans) likely mediate tubulin interaction, though direct structural data for LinJ25.1190 remains unpublished .
Enzymatic Activity
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Acetylates α-tubulin at K40 during microtubule polymerization .
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Activity validated via SDS-PAGE and Western blot using acetylated tubulin antibodies .
In Vitro Studies
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Tubulin acetylation assays: Used to probe microtubule dynamics in protozoan parasites .
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Enzyme kinetics: Comparative studies with human αTAT1 to identify species-specific regulatory mechanisms .
Therapeutic Relevance
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Parasite biology: K40 acetylation influences Leishmania motility and infectivity, making this enzyme a potential drug target .
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Neurological models: Human homolog αTAT1 rescues axon growth in CSPG/MAG-inhibited neurons, suggesting conserved functional roles .
Comparative Analysis
| Feature | LinJ25.1190 (Leishmania) | Human αTAT1 |
|---|---|---|
| Catalytic residues | Undetermined | R69, H75, K102 |
| Acetylation site | α-tubulin K40 | α-tubulin K40 |
| Pathway regulation | RhoA-ROCK (inferred) | RhoA-ROCK confirmed |
Technical Notes
Product Specs
Target Background
KEGG: lif:LINJ_25_1190
