Recombinant Anabaena variabilis Protease HtpX homolog (htpX)
Description
Definition and Production
Recombinant Anabaena variabilis Protease HtpX homolog (htpX) is a full-length, His-tagged protein (UniProt ID: Q3MH22) expressed in Escherichia coli. It consists of 289 amino acids (MW: ~33 kDa) and is purified to >90% purity via affinity chromatography . Key features include:
Biochemical Properties
Key functional attributes include:
Functional Roles
HtpX homologs are implicated in:
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Membrane protein quality control: Complements ATP-dependent proteases like FtsH in degrading misfolded membrane proteins .
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Regulated intramembrane proteolysis (RIP): Cleaves transmembrane segments of substrates to release transcription factors or signaling molecules .
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Stress adaptation: Likely involved in Anabaena’s differentiation processes (e.g., heterocyst formation) .
Protease Activity and Mechanism
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Mutagenesis of the HEXXH motif (e.g., E192Q) abolishes activity, confirming metalloprotease dependence on zinc coordination .
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Cleaves artificial substrates (e.g., pro-σK) at specific glycine-tyrosine bonds, analogous to Bacillus subtilis SpoIVFB .
Localization and Expression
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Homologs in E. coli and B. subtilis are plasma membrane-integrated with cytosolic active sites .
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In Anabaena, HtpX-related proteases are linked to nitrogen fixation and stress responses .
Applications
Product Specs
Note: While we strive to ship the format currently in stock, we are happy to accommodate specific requests. Please indicate your desired format in the order notes and we will fulfill your preference whenever possible.
Note: All protein shipments are standardly packaged with blue ice packs. If dry ice packaging is required, please contact us in advance for arrangement and associated fees.
Generally, liquid form has a shelf life of 6 months at -20°C/-80°C. Lyophilized form maintains a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: ava:Ava_0088
STRING: 240292.Ava_0088
