Recombinant Anguilla anguilla Hemoglobin cathodic subunit beta (hbb2)
Shipped with Ice Packs 
In Stock 
Description
Functional Characteristics
Recombinant hbb2 demonstrates oxygen-binding properties modulated by organic phosphates and pH:
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GTP Sensitivity: GTP reduces oxygen affinity by 70% and eliminates the reverse Bohr effect (where oxygen affinity increases with acidity) .
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Root Effect: Acidification induces oxygen release (PO₂ increases by 7–8× in rete mirabile), critical for swimbladder gas secretion .
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CO₂ Transport: Aquaporins in gas gland cells facilitate CO₂ diffusion, enhancing proton release and Root effect activation .
Key functional metrics:
Genomic and Evolutionary Context
hbb2 is part of a teleost-specific hemoglobin diversification driven by whole-genome duplication (WGD):
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Gene Clusters: Teleost hemoglobin genes reside in two chromosomal regions:
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Evolutionary Adaptations:
Research Applications
Recombinant hbb2 is used to study:
Product Specs
Form
Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipment, contact us in advance; additional fees apply.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receiving. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag type, please inform us, and we will prioritize developing it.
Synonyms
hbb2; Hemoglobin cathodic subunit beta; Hemoglobin cathodic beta chain
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-146
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Anguilla anguilla (European freshwater eel) (Muraena anguilla)
Target Names
hbb2
Target Protein Sequence
VEWSASERST ITSLWGKINV AEIGPQALAR VLIVYPWTQR YFGKFGDLSN AAAIQGNAKV
AAHGKVVLGA LEKAVKNMDD VKGTYSKLSQ LHNEKLNVDP DNFRLLGDCL TIVLATKLGA
GFPAEIQAVW QKFVAVVVSA LSKQYF
Uniprot No.
Target Background
Function
Involved in oxygen transport from the gills to peripheral tissues.
Protein Families
Globin family
Tissue Specificity
Red blood cells.
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