Recombinant Anolis carolinensis Rhodopsin (RHO)
Description
Introduction to Recombinant Anolis carolinensis Rhodopsin (RHO)
Recombinant Anolis carolinensis rhodopsin refers to a genetically engineered version of the rhodopsin protein found in the green anole lizard, Anolis carolinensis. Rhodopsin is a crucial protein for vision, particularly in low-light conditions, and is encoded by the RHO gene. In Anolis carolinensis, rhodopsin has been detected primarily in the pineal gland, which plays a role in controlling circadian rhythms .
Function and Structure of Rhodopsin
Rhodopsin is a G-protein-coupled receptor (GPCR) that triggers visual phototransduction in rod cells. It consists of a protein component called opsin and a vitamin A-derived chromophore, 11-cis retinal. When light is absorbed, 11-cis retinal isomerizes to all-trans retinal, initiating a signaling cascade that leads to visual perception . The structure of rhodopsin includes seven transmembrane domains, with the retinal binding site located in the seventh domain .
Recombinant Rhodopsin Production
Recombinant rhodopsin is produced through genetic engineering techniques where the RHO gene from Anolis carolinensis is cloned and expressed in a suitable host system, such as bacteria or mammalian cells. This allows for the large-scale production of the protein for research and potential therapeutic applications.
Research Findings on Anolis carolinensis Rhodopsin
Studies on the rhodopsin of Anolis carolinensis have identified several amino acid substitutions unique to this species. These substitutions, such as S22N, N199H, E232A, and T319M, are located outside the transmembrane domains and may be crucial for its function in the pineal gland . The specific role of these substitutions in circadian rhythm regulation is an area of ongoing research.
Data and Tables
While specific data tables for recombinant Anolis carolinensis rhodopsin are not readily available, the following table summarizes key features of rhodopsin in general and highlights the unique aspects of Anolis carolinensis rhodopsin:
| Feature | General Rhodopsin | Anolis carolinensis Rhodopsin |
|---|---|---|
| Location | Rod cells in retina | Primarily in pineal gland |
| Function | Low-light vision | Circadian rhythm regulation |
| Structure | 7 transmembrane domains | Similar structure with unique substitutions |
| Unique Substitutions | None noted | S22N, N199H, E232A, T319M |
Product Specs
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Target Background
STRING: 28377.ENSACAP00000014023
