Recombinant Anoxybacillus flavithermus UPF0344 protein Aflv_2205 (Aflv_2205)
Description
Production and Purification
The protein is produced in Escherichia coli expression systems, followed by affinity chromatography using the His-tag. Critical production parameters include:
Table 2: Production and Quality Control
Functional and Genomic Context
While functional data for Aflv_2205 is limited, comparative genomic studies of A. flavithermus suggest potential roles in stress adaptation or membrane-associated processes in thermophilic environments . Notably:
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A. flavithermus strains harbor alkali-tolerant genes (e.g., Na+/H+ antiporters) that stabilize proteins under extreme conditions .
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The Aflv_2205 gene is distinct from well-characterized thermophilic enzymes like AnoCas9, a CRISPR-associated protein studied for its compact nuclease activity .
Applications and Research Utility
Recombinant Aflv_2205 is primarily used in:
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Structural Studies: Investigating transmembrane protein dynamics due to its predicted membrane localization .
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Thermophilic Mechanism Research: Serving as a model for protein stability in high-temperature environments .
Limitations and Future Directions
Current knowledge gaps include:
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Functional Pathways: No confirmed metabolic or signaling pathways linked to Aflv_2205 .
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Interaction Networks: Direct protein-protein interactions remain uncharacterized . Further studies using structural biology (e.g., cryo-EM) and knockout models are needed to elucidate its biological significance.
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
KEGG: afl:Aflv_2205
STRING: 491915.Aflv_2205
