Recombinant Arabidopsis thaliana B3 domain-containing protein REM17 (REM17), partial
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Target Background
Q&A
Basic Research Questions
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What is the REM gene family in Arabidopsis thaliana and how is it structured?
The REM gene family of Arabidopsis thaliana comprises 45 genes that are part of the B3 DNA-binding domain superfamily . These genes are phylogenetically divergent, extensively duplicated, and mostly located in clusters throughout the Arabidopsis genome . REM proteins contain the B3 DNA-binding domain in variable numbers (one to seven copies per protein). The family has undergone significant duplication events, leading to functional redundancy among closely related members . REM genes are primarily expressed during flower and seed development, suggesting their critical roles in reproductive development .
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What are the characteristic structural features of REM proteins?
REM proteins are characterized by the presence of B3 DNA-binding domains. These domains may appear in variable numbers within a single protein, with some members containing as few as one and others having up to seven copies . Despite belonging to the same family, REM proteins show sequence divergence even within the B3 DNA-binding domain . Most REM proteins likely function as transcription factors, with evidence suggesting that at least some members (like REM34) act as transcriptional activators . The variations in B3 domain organization likely contribute to different DNA-binding specificities and functional properties among family members.
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What expression patterns are typically observed for REM family members?
REM genes show distinct expression patterns primarily associated with reproductive development. Many REM genes, including the well-studied REM34, REM35, and REM36, are expressed in:
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Inflorescence meristems
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Flower meristems
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Early stages of flower development
Specifically, REM34, REM35, and REM36 show expression in:
Most REM genes are notably absent in vegetative tissues and sepals, underscoring their specialized roles in reproductive development .
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How are REM genes organized genomically in Arabidopsis thaliana?
REM genes are predominantly arranged in clusters within the Arabidopsis genome . The largest cluster is located on chromosome 4, containing nine REM genes (REM34, REM35, REM36, REM37, REM38, REM39, REM40, REM41, and REM42) within a 30 kb region . This cluster is partially duplicated on chromosome 2 (REM29, REM30, REM31, and REM32) . This genomic organization with close linkage of related REM genes creates challenges for traditional knockout approaches, as generating multiple mutants through crossing is practically impossible for linked genes . Researchers should consider this genomic arrangement when designing experimental strategies for studying REM17 and other family members.
