Recombinant Arabidopsis thaliana Delta-9 desaturase-like 2 protein (At1g06100)

Overview of Recombinant Arabidopsis thaliana Delta-9 Desaturase-like 2 Protein (At1g06100)

Arabidopsis thaliana Delta-9 desaturase-like 2 protein (At1g06100) is a member of the acyl-coenzyme A (CoA) desaturase-like (ADS) gene family in Arabidopsis thaliana . This family comprises nine genes that encode fatty acid desaturase-like proteins .

Characteristics

• Synonyms: At1g06100, T21E18.15, T21E18_12, Delta-9 desaturase-like 2 protein

• Source: Produced in an in vitro E. coli expression system

• Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein

• Protein Length: Full length protein, with the expression region spanning from 1-299

• Function: Exhibits desaturase activity on very-long-chain fatty acid (VLCFA) substrates, introducing double bonds at the n-7 position relative to the methyl end of the molecule

Function and Activity

At1g06100, also referred to as AtADS1.3, demonstrates desaturase activity by introducing double bonds at the n-7 position on very-long-chain fatty acid (VLCFA) substrates . While only one member of the Arabidopsis thaliana acyl-coenzyme A (CoA) desaturase-like (ADS) gene family, fatty acid desaturase5 (AtADS3/FAD5, At3g15850), has a known biological function, research has shown that At1g06100 has previously undescribed desaturase activity .

Genetic and Expression Details

• Gene Family: Belongs to the fatty acid desaturase type 1 family

• Database Links: KEGG (ath:AT1G06100), STRING (3702.AT1G06100.1), UniGene (At.50644)

• The Arabidopsis thaliana genome contains nine ADS Δ9 desaturase family members .

Role in Fatty Acid Metabolism

AtADS2 is involved in synthesizing the 24:1(n-9) and 26:1(n-9) components of seed lipids, sphingolipids, and membrane phospholipids like phosphatidylserine and phosphatidylethanolamine . Plants with deficient AtADS2 expression exhibit a near complete loss of phosphatidylethanolamine(42:4), phosphatidylserine(42:4), dihydroxy-monohexosylceramide(42:2)-2, trihydroxy-monohexosylceramide(42:2)-3, and trihydroxy-glycosylinositolphosphoceramide(42:2)-3, lipid species that contain the VLCFA 24:1(n-9), and trihydroxy-glycosylinositolphosphoceramide(44:2)-3, a lipid containing 26:1(n-9) . Acyl-CoA profiling of these plants showed a major reduction in 24:1-CoA and a slight reduction in 26:1-CoA . Overexpression of AtADS2 leads to a substantial increase in the percentage of glycerolipid and sphingolipids species containing 24:1 and a dramatic increase in the percentage of very-long-chain monounsaturated fatty acids in the acyl-CoA pool .