Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase At4g11680 (At4g11680)
Description
Protein Overview
Recombinant At4g11680 belongs to the RING-type E3 ubiquitin ligase family, which facilitates substrate-specific ubiquitination by transferring ubiquitin from E2 conjugating enzymes to target proteins. Key features include:
| Attribute | Details |
|---|---|
| Gene Name | At4g11680 |
| Synonyms | RING finger protein At4g11680; T5C23.110 |
| Species | Arabidopsis thaliana (Mouse-ear cress) |
| Protein Length | Full-length (1-390 amino acids) |
| Molecular Weight | ~44 kDa (calculated) |
| Tag | N-terminal His-tag |
| Expression System | Escherichia coli |
| Purity | >90% (SDS-PAGE verified) |
Amino Acid Sequence
The protein sequence begins with:
MSSSSSTTTNTTTESDSSSLPTHIGRSNSDGIIDTTPFLPPTVTRTISVDEESNPIHRSA...
Key domains include:
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RING Finger Domain: Critical for E2 binding and ubiquitin transfer activity.
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Transmembrane Regions: Predicted hydrophobic segments (e.g., residues 150-170) suggest membrane association .
Quality Control
Functional Significance in Plant Biology
E3 ligases like At4g11680 regulate diverse cellular processes:
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Abiotic Stress Responses: Related RING-type ligases (e.g., AtCHIP) mediate thermotolerance and drought resistance by degrading misfolded proteins .
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Circadian Rhythms: Homologs such as AtSINAL7 exhibit circadian expression patterns, linking ubiquitination to developmental timing .
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Hormone Signaling: E3 ligases modulate abscisic acid (ABA) pathways, affecting seed dormancy and stress adaptation .
Experimental Uses
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Ubiquitination Assays: Study substrate specificity and enzyme kinetics .
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Protein-Protein Interaction Screens: Identify binding partners via His-tag pulldowns .
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Stress Response Studies: Investigate roles in heat, salinity, or pathogen resistance .
Key Findings from Related Studies
Product Specs
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Target Background
Mediates E2-dependent protein ubiquitination in vitro.
