Recombinant Arabidopsis thaliana MLO-like protein 10 (MLO10)
Description
Introduction to Recombinant Arabidopsis thaliana MLO-like Protein 10 (MLO10)
Recombinant Arabidopsis thaliana MLO-like protein 10 (MLO10) is a protein derived from the model plant Arabidopsis thaliana. MLO proteins are part of a large family of proteins known for their roles in plant defense mechanisms and reproductive processes. Specifically, MLO10 has been identified as a key component in pollen-stigma recognition and pollen tube elongation during plant reproduction.
Biological Function of MLO10
MLO10 interacts with another protein, CML9 (Calcium/Calmodulin-like protein 9), to form a complex that plays a crucial role in pollen germination and pollen tube elongation. This interaction is essential for successful fertilization in plants. Studies using Arabidopsis thaliana have shown that mutations in either MLO10 or CML9 lead to reduced pollen germination rates and shorter pollen tubes, highlighting their importance in plant reproductive processes .
Expression and Localization
MLO10 is expressed in the stigma of Arabidopsis thaliana, while CML9 is expressed in pollen and pollen tubes. The interaction between these two proteins is believed to facilitate pollen-stigma recognition, which is a critical step in plant reproduction. The specific expression patterns suggest a maternal-paternal interaction model, where MLO10 in the stigma interacts with CML9 from the pollen .
Recombinant Production of MLO10
Recombinant MLO10 proteins can be produced in various expression systems, including yeast, E. coli, baculovirus, and mammalian cells. These recombinant proteins are useful for studying the biochemical properties of MLO10 and its interactions with other proteins like CML9. For example, biotinylated versions of MLO10 can be produced using AviTag-BirA technology in E. coli .
Research Findings
Recent studies have focused on understanding the role of MLO10 in plant reproduction. Key findings include:
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Pollen Germination and Tube Elongation: Mutations in MLO10 or CML9 significantly reduce pollen germination rates and pollen tube lengths, affecting seed setting .
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Protein Interaction: The interaction between MLO10 and CML9 is specific and involves the C-terminal region of MLO10, particularly the P5 domain .
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Reproductive Physiology: The MLO10-CML9 complex is crucial for pollen-stigma recognition and subsequent fertilization processes .
Table 1: Expression Systems for Recombinant MLO10
| Code | Source | Description |
|---|---|---|
| CSB-YP863793DOA1 | Yeast | Recombinant MLO10 protein |
| CSB-EP863793DOA1 | E. coli | Recombinant MLO10 protein |
| CSB-EP863793DOA1-B | E. coli | Biotinylated recombinant MLO10 protein |
| CSB-BP863793DOA1 | Baculovirus | Recombinant MLO10 protein |
| CSB-MP863793DOA1 | Mammalian cell | Recombinant MLO10 protein |
Table 2: Effects of MLO10 and CML9 Mutations on Pollen Germination and Tube Elongation
| Mutation | Pollen Germination Rate (%) | Pollen Tube Length |
|---|---|---|
| Col-0 (Wild-type) | 75% | Normal length |
| cml9-1 | 60% | Shorter |
| cml9-2 | 60% | Shorter |
Product Specs
Please note: We will prioritize shipping the format currently in stock. However, if you have a specific format preference, please specify your requirements when placing the order, and we will fulfill your request to the best of our ability.
Note: All protein shipments are standardly packaged with blue ice packs. If you require dry ice packaging, please inform us in advance. Additional fees may apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
