Recombinant Arabidopsis thaliana Peroxisomal membrane protein 11D (PEX11D)
Description
Introduction to Recombinant Arabidopsis thaliana Peroxisomal Membrane Protein 11D (PEX11D)
Recombinant Arabidopsis thaliana Peroxisomal membrane protein 11D (PEX11D) is a member of the PEX11 protein family, which plays a crucial role in peroxisome proliferation and dynamics in plants. Peroxisomes are vital organelles involved in various metabolic processes, including fatty acid oxidation and the detoxification of reactive oxygen species. The PEX11 family consists of several isoforms, including PEX11A, PEX11B, PEX11C, PEX11D, and PEX11E, each exhibiting distinct functions in peroxisome biogenesis and maintenance.
Role of PEX11D in Peroxisome Dynamics
The primary function of PEX11D is to promote peroxisome proliferation through mechanisms involving membrane elongation and fission. Studies have shown that all members of the PEX11 family contribute to these processes by interacting with other proteins that mediate peroxisome division .
Experimental Findings
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Overexpression Studies: In Arabidopsis thaliana, overexpression of PEX11D resulted in significant changes to peroxisome morphology, indicating its role as a membrane elongation factor .
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Gene Expression Analysis: Reduction of PEX11D expression led to decreased peroxisome abundance, highlighting its importance in maintaining peroxisome populations within plant cells .
Comparative Analysis of PEX11 Family Members
| Protein | Role in Peroxisome Proliferation | Complementation of Yeast Mutants |
|---|---|---|
| PEX11A | Integral membrane protein; promotes elongation | Limited complementation |
| PEX11B | Integral membrane protein; less characterized | Not effective |
| PEX11C | Integral membrane protein; significant elongation | Strong complementation |
| PEX11D | Integral membrane protein; promotes elongation | Moderate complementation |
| PEX11E | Integral membrane protein; significant elongation | Strong complementation |
This table summarizes the roles of different PEX11 proteins in peroxisome dynamics and their ability to complement yeast mutants deficient in peroxisome function.
References
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Travis Orth et al., "The PEROXIN11 Protein Family Controls Peroxisome Proliferation in Arabidopsis," The Plant Cell, Volume 19, Issue 1, January 2007.
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Sigrun Reumann et al., "PEX11 family members are membrane elongation factors," Journal of Cell Science, 2010.
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Lingard et al., "Overexpression of At PEX11 genes induces peroxisome proliferation," Plant Physiology, 2006.
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Mathur et al., "High-resolution imaging of peroxisomes," Journal of Cell Science, 2002.
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Fan et al., "Fluorescence microscopy analysis of Arabidopsis peroxisomes," Plant Cell, 2005.
Product Specs
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Target Background
Involved in peroxisomal proliferation. It promotes peroxisomal duplication, aggregation, or elongation without fission.
- Research suggests a model for peroxisome replication where PEX11c, PEX11d, and PEX11e cooperate during the G2 phase to promote peroxisome elongation and recruit FIS1b to the peroxisome membrane. PMID: 18539750
