Recombinant Arabidopsis thaliana Putative cyclic nucleotide-gated ion channel 15 (CNGC15)
Description
Functional Roles in Plant Physiology
CNGC15 exhibits dual roles in calcium signaling and nitrate response, with subcellular localization dependent on developmental stage:
Nuclear Calcium Signaling
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Mediates nuclear calcium release in root apical meristem cells, essential for root development in young seedlings .
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Interacts with AtDMI1 at the nuclear envelope to regulate auxin homeostasis .
Nitrate Signaling
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Relocalizes to the plasma membrane in older roots to form a complex with nitrate transceptor NRT1.1 .
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Modulates nitrate-induced expression of LBD37, LBD38, and LBD39 genes, which regulate lateral root development .
Mutant Allele Studies
A novel mutant allele (Atcngc15-D427N) with a conserved aspartic acid mutation (D427N) in the C-linker domain shows:
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Impaired channel gating, leading to reduced nuclear calcium flux .
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Shortened root apical meristem and disrupted LBD39 induction under nitrate treatment .
Subcellular Dynamics
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Early development (6 days): Localizes to the nuclear envelope .
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Later stages (12 days): Translocates to the plasma membrane in columella cells under nitrate exposure .
Interaction Network
| Interacting Partner | Function | Reference |
|---|---|---|
| NRT1.1 | Nitrate sensing and transport | |
| AtDMI1 | Nuclear calcium oscillations | |
| CPK10/30/32 | Calcium-dependent kinase signaling |
Mechanistic Studies
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Used to dissect calcium signaling pathways in root development and nitrate responses .
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Serves as a model for studying CNGC channel gating mechanisms .
Agricultural Potential
Product Specs
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Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
