Recombinant Arabidopsis thaliana Putative UDP-glucuronate:xylan alpha-glucuronosyltransferase 4 (GUX4)

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Cat. No.

BT2498805

Source

in vitro E.coli expression system

Synonyms

GUX4; PGSIP4; At1g54940; F14C21.47; T24C10.6; Putative UDP-glucuronate:xylan alpha-glucuronosyltransferase 4; UDP-GlcA:xylan glucuronyltransferase 4; Glycogenin-like protein 4; Plant glycogenin-like starch initiation protein 4; Protein GLUCURONIC ACID SUBSTITUTION OF XYLAN 4; AtGUX4

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Description

Introduction to Recombinant Arabidopsis thaliana Putative UDP-glucuronate:xylan alpha-glucuronosyltransferase 4 (GUX4)

Recombinant Arabidopsis thaliana Putative UDP-glucuronate:xylan alpha-glucuronosyltransferase 4 (GUX4) is an enzyme involved in the synthesis of xylan, a major component of plant cell walls. Xylan is crucial for plant structure and function, particularly in secondary cell walls. GUX4 belongs to the Glycosyltransferase Family 8 and plays a role in adding glucuronic acid substitutions to the xylan backbone, which is essential for the structural integrity and functionality of plant cell walls.

Role of GUX4 in Xylan Synthesis

GUX4 is one of several enzymes responsible for modifying xylan by adding glucuronic acid residues. This process is vital for the proper assembly and function of plant cell walls. The enzyme works by transferring glucuronic acid from UDP-glucuronic acid to specific xylose residues on the xylan backbone. This modification affects the physical properties of xylan and its interactions with other cell wall components.

Research Findings and Implications

Research on GUX4 and related enzymes has highlighted their importance in plant cell wall biosynthesis. Studies have shown that mutations or alterations in these enzymes can affect plant growth and cell wall composition. For example, mutations in GUX1 and GUX2 have been shown to significantly reduce the glucuronic acid content in xylan, impacting plant development .

Table 1: Comparison of GUX Enzymes Involved in Xylan Synthesis

Enzyme	Localization	Substrate Specificity	Activity
GUX1	Golgi	Xylohexaose	High
GUX2	Golgi	Xylohexaose	High
GUX4	Golgi	Xylohexaose	Active

Product Specs

Form

Lyophilized powder
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Lead Time

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Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, which can be used as a reference.

Shelf Life

Shelf life depends on storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during the manufacturing process.
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Synonyms

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-557

Protein Length

full length protein

Species

Arabidopsis thaliana (Mouse-ear cress)

Target Names

GUX4

Target Protein Sequence

MGTKTHNSRGKIFMIYLILVSLSLLGLILPFKPLFRITSPSSTLRIDLPSPQVNKNPKWL RLIRNYLPEKRIQVGFLNIDEKERESYEARGPLVLKNIHVPLDHIPKNVTWKSLYPEWIN EEASTCPEIPLPQPEGSDANVDVIVARVPCDGWSANKGLRDVFRLQVNLAAANLAVQSGL RTVNQAVYVVFIGSCGPMHEIFPCDERVMRVEDYWVYKPYLPRLKQKLLMPVGSCQIAPS FAQFGQEAWRPKHEDNLASKAVTALPRRLRVAYVTVLHSSEAYVCGAIALAQSIRQSGSH KDMILLHDHTITNKSLIGLSAAGWNLRLIDRIRSPFSQKDSYNEWNYSKLRVWQVTDYDK LVFIDADFIILKKLDHLFYYPQLSASGNDKVLFNSGIMVLEPSACMFKDLMEKSFKIESY NGGDQGFLNEIFVWWHRLSKRVNTMKYFDEKNHRRHDLPENVEGLHYLGLKPWVCYRDYD CNWDISERRVFASDSVHEKWWKVYDKMSEQLKGYCGLNKNMEKRIEKWRRIAKNNSLPDR HWEIEVRDPRKTNLLVQ

Uniprot No.

Q9FZ37

Target Background

Function

Putative involvement in xylan backbone substitution within stem glucuronoxylan.

Gene References Into Functions

GUX1, GUX2, and GUX4 exhibit activity as xylan alpha-glucuronosyltransferases. [GUX4] PMID: 22706449

Database Links

KEGG: ath:AT1G54940

STRING: 3702.AT1G54940.1

Protein Families

Glycosyltransferase 8 family, Glycogenin subfamily

Subcellular Location

Golgi apparatus membrane; Single-pass type II membrane protein.

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Recombinant Arabidopsis thaliana Putative UDP-glucuronate:xylan alpha-glucuronosyltransferase 4 (GUX4)

Description

Introduction to Recombinant Arabidopsis thaliana Putative UDP-glucuronate:xylan alpha-glucuronosyltransferase 4 (GUX4)

Role of GUX4 in Xylan Synthesis

Research Findings and Implications

Table 1: Comparison of GUX Enzymes Involved in Xylan Synthesis

Product Specs

Target Background

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