Recombinant Archaeoglobus fulgidus Uncharacterized protein AF_1283 (AF_1283)
Description
Basic Characteristics of AF_1283
AF_1283 is encoded by the gene AF_1283 in A. fulgidus (UniProt ID: O28985) and belongs to a family of uncharacterized proteins. Key attributes include:
Amino Acid Sequence
The full-length protein (1–100 aa) includes hydrophobic regions (e.g., LIVGLLFGLSG) and potential β-strand/α-helix domains. The partial version (CSB-MP518092DOC1) lacks complete structural data .
Molecular Weight
While not explicitly stated, the sequence length (100 aa) suggests a molecular weight of ~11 kDa (average aa molecular weight = 110 Da).
Production and Quality Control
Recombinant AF_1283 is produced via heterologous expression systems, ensuring high purity and scalability:
| Parameter | E. coli Expression (Full-Length) | Mammalian Cell Expression (Partial) |
|---|---|---|
| Tag | His-tag | Undisclosed |
| Purity Method | SDS-PAGE (>90%) | SDS-PAGE (>85%) |
| Yield | Lyophilized powder | Lyophilized or liquid |
| Storage Buffer | Tris/PBS-based, 6% trehalose, pH 8.0 | Not specified |
Research Applications and Potential
While AF_1283’s biological role is uncharacterized, its recombinant form enables:
-
Structural Studies: Crystallization and NMR/EM analysis to resolve 3D conformation.
-
Functional Screening: Biochemical assays to identify interactions (e.g., DNA binding, enzyme activity).
-
Pathway Mapping: Co-expression with archaeal proteins to infer participation in metabolic or stress-response pathways.
Current Research Gaps
-
Functional Annotation: No studies directly link AF_1283 to specific processes (e.g., DNA repair, metabolism).
-
Interaction Partners: Limited data on binding partners or cofactors.
-
In Vivo Relevance: Role in A. fulgidus’ adaptation to hyperthermophilic environments remains speculative.
Product Specs
Note: While we prioritize shipping the format currently in stock, we can accommodate specific format requirements. Please indicate your preference in the order notes, and we will prepare accordingly.
Note: All protein shipments are standardly packaged with blue ice packs. If dry ice shipping is required, please contact us in advance. Additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: afu:AF_1283
STRING: 224325.AF1283
