Recombinant Archaeoglobus fulgidus Uncharacterized protein AF_2028 (AF_2028)

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Cat. No.

BT2475574

Source

in vitro E.coli expression system

Synonyms

AF_2028; Uncharacterized protein AF_2028

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Description

General Information

Recombinant Archaeoglobus fulgidus Uncharacterized Protein AF_2028 (AF_2028) is a protein derived from the archaeon Archaeoglobus fulgidus . It is produced using recombinant DNA technology . AF_2028 is referred to as an uncharacterized protein, which indicates that its specific function has not yet been determined through experimentation .

Table 1: General Information of Recombinant AF_2028

Feature	Description
Species	Archaeoglobus fulgidus
Source	E. coli
Tag	His-Tag
Protein Length	Full Length (1-607 amino acids)
Form	Lyophilized powder
Purity	Greater than 90% as determined by SDS-PAGE
UniProt ID	O28251
Synonyms	AF_2028, Uncharacterized protein AF_2028
Gene Name	AF_2028

Production and Characteristics

The AF_2028 protein is produced in E. coli cells as a recombinant protein with an N-terminal His tag to facilitate purification . The protein is available as a lyophilized powder . The purity is assessed using SDS-PAGE, with a reported purity level of greater than 90% .

Table 2: Production and Biochemical Details

Parameter	Description
Expression System	E. coli
Tag	N-terminal His tag
Molecular Weight	Reportedly 68.2 kDa based on the number of amino acids.
Purity	>85% (SDS-PAGE)
Stability	Stable for 6 months in liquid form or 12 months in lyophilized form when stored at -20°C/-80°C

Biological Context and Potential Functions

AF_2028 is derived from Archaeoglobus fulgidus, a hyperthermophilic archaeon known for its ability to reduce sulfate at high temperatures . While AF_2028 is currently annotated as an uncharacterized protein, studies on A. fulgidus have revealed insights into its stress response mechanisms and other cellular processes .

Although the specific function of AF_2028 remains unknown, several lines of evidence suggest potential roles:

Involvement in Cellular Pathways: AF_2028 may participate in various cellular pathways and functions, possibly in conjunction with other proteins .
Response to Stress: As a product of a hyperthermophilic archaeon, AF_2028 might play a role in the organism's response to heat and other environmental stresses .

Product Specs

Form

Lyophilized powder.
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for fulfillment.

Lead Time

Delivery times vary depending on the purchase method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a guideline.

Shelf Life

Shelf life depends on storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.

Storage Condition

Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.

Tag Info

The tag type is determined during the manufacturing process.
Note: While the tag type is determined during production, we prioritize fulfilling specific tag requests if provided at the time of order.

Synonyms

AF_2028; Uncharacterized protein AF_2028

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-607

Protein Length

full length protein

Species

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Target Names

AF_2028

Target Protein Sequence

MLITITSMSAGRLGKIGLVAMIIVLTIITINTSAAPLEVSIEERVNSTINMTDAANGVGT FEYQTDVKGIINVTNNANEDIMDIWVAVDIGLNDGSCSAISTGVQILDSGDVPSKLTNEG GFDTSGADCIIHIPRLGPGESKYVAYDVTETPEIQNGAPFIVEEKYDPAKIPKGGDYTWT VYFNVSLNDTWWSSTGLGGKPSSVTLTVNKNLNTSYDTNWKTLEFADGSSNPKQIISGTL DNNKWLTTTFKVKGNYSTDEAFVSRLVGFGEAYFQFGPINGNISGTKIIDVFAIGNASIG VNKSGPDNSNQWTGNVTIKNTATGLTYIVKSVKVWATDRNYNEINGARYENTTVNVQIGR DESFTSKDLSFQYDKVPIIWGNVTFRLVEDANYGWGVGQDKITDGGNTYIIERIYVIGSY LVKVTKHVESAGNDIYNITLVVENLGGQESPYVYVYDLIPKNFSLTNGDNDWKDPQDRDG DWVNKSSMLAGGPETITNIQLSGYDTGYWWRIRPINASADGDGAYDDYTEIENNQTVVIF YQIQGSEDYKLLDAFIVGIDPILSMNEQTSPKITLVSGAKATSYESVMALATALVGLTAV VGYRRRS

Uniprot No.

O28251

Target Background

Database Links

KEGG: afu:AF_2028

STRING: 224325.AF2028

Subcellular Location

Cell membrane; Multi-pass membrane protein.

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Recombinant Archaeoglobus fulgidus Uncharacterized protein AF_2028 (AF_2028)

Description

General Information

Table 1: General Information of Recombinant AF_2028

Production and Characteristics

Table 2: Production and Biochemical Details

Biological Context and Potential Functions

Product Specs

Target Background

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