Recombinant Arthrobacter sp. Undecaprenyl-diphosphatase (uppP)
Description
Enzymatic Function and Biological Role
UppP is an integral membrane protein responsible for maintaining UP pools required for:
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Peptidoglycan synthesis: UP transports precursors like N-acetylglucosamine and N-acetylmuramic acid across the membrane .
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Cell wall homeostasis: Depletion of UP disrupts lipid II cycling, leading to cell lysis or morphological defects .
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Antibiotic resistance: UppP activity influences susceptibility to bacitracin, which sequesters UPP .
In Bacillus subtilis, UppP works synergistically with BcrC phosphatase, forming a synthetic lethal pair essential for sporulation and vegetative growth .
Recombinant Production Strategies
While no published data explicitly details Arthrobacter sp. UppP production, analogous systems suggest:
For Arthrobacter sp. enzymes, secretion systems improve solubility, and codon-optimized genes enhance expression .
Biochemical Properties
Homologous UppP enzymes exhibit:
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Temperature stability: Activity retained at 20–25°C, with reduced function at extremes .
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Substrate specificity: Strict preference for UPP; no activity toward unrelated pyrophosphates .
Functional Insights from Mutagenesis
Key structural motifs identified in Escherichia coli UppP include:
Deletion of uppP in B. subtilis results in:
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Sporulation defects: <7% sporulation efficiency vs. 30% in wild-type .
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Cell elongation: Filamentous growth under UPP phosphatase limitation .
Therapeutic and Industrial Relevance
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Antibiotic targeting: UppP inhibition disrupts cell wall synthesis, making it a potential drug target .
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Biotechnological applications: Recombinant UppP could optimize glycan synthesis pathways in engineered microbes .
Current Research Gaps
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it in your order notes. We will accommodate your request whenever possible.
Note: All protein shipments default to blue ice packs. If dry ice shipping is required, please inform us in advance as additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: art:Arth_2167
STRING: 290399.Arth_2167
