Recombinant Ashbya gossypii 3-keto-steroid reductase (ERG27)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we will prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a guideline.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The specific tag type is finalized during production. If a particular tag type is required, please inform us, and we will prioritize its incorporation.
Synonyms
ERG27; AGR068W; 3-keto-steroid reductase; EC 1.1.1.270
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-348
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Target Names
ERG27
Target Protein Sequence
MLICHQCLSS RLTYISNLGL NIAYRLIEQF TDDSKLVIVV TSRTLPRVRE VVDLIKTYAE
KCGKSGAVDF DYLLVDFTDM VSVLGAAYEL EKRYDAIHYF YANAAQGVYS GIDWLGATKA
VLRDPLDAVT NPTYKIQRVG VKSRDGLGLV FQANVFGPYY LIRRIIPLLA KGKAKVVWLS
SLMSDVKYLS LEDVELLRTD SSYEGSKRLV DLLHLATYKE LKSLGIHQYV THPGIFTSHS
FYQYLNFFTY YGMLFLFYLA RWLGSPWHNI QGYKGANAPI YVATLANPTF EHQALKYGSA
TYRDGMEYIA KHEIDPTGMH DAYKYIRDLA EEWDIKLKDQ ITIGRSLS
Uniprot No.
Target Background
Function
This enzyme is responsible for the reduction of the keto group at the C-3 position of sterols.
Database Links
KEGG: ago:AGOS_AGR068W
STRING: 33169.AAS54557
Protein Families
Short-chain dehydrogenases/reductases (SDR) family, ERG27 subfamily
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