Recombinant Asterias rubens Cytidine monophosphate-N-acetylneuraminic acid hydroxylase (cnh)
Description
Introduction to Recombinant Asterias rubens Cytidine Monophosphate-N-Acetylneuraminic Acid Hydroxylase
Recombinant Asterias rubens Cytidine monophosphate-N-acetylneuraminic acid hydroxylase, commonly referred to by its gene name "cnh," is an enzyme crucial for the synthesis of sialic acids, specifically N-glycolylneuraminic acid (Neu5Gc) from CMP-N-acetylneuraminic acid (CMP-Neu5Ac). This enzyme is particularly significant in echinoderms, where Neu5Gc is a predominant form of sialic acid. The recombinant form of this enzyme is produced in E. coli and is His-tagged for easier purification and identification.
2.3. Amino Acid Sequence
The amino acid sequence of the recombinant enzyme is provided by Creative Biomart and includes 653 amino acids starting with MEQEREIVFSLSPEETSELKNGVNLISRSEKEKFVIYKDPTAENTVEEPATSHMYKACLNKCKHQGGTFIKDIEDGDNCILRCTKHGWKLDAKTMRYVNPPDSFSQQQLVPEYNEDGSLD IVELKPPQPWETDKRDPMPLEVGEVQITYFTHACIEIKLGDLIMFTDPWLIGPAFARGWW LMHEPPADWLDRLAKADLIYISHLHSDHLNYPTLELLSQRNPDIPIYVGDTSMPVFVRLE QSGVKLNNIHIKKFGKWIEINKDTRFMIMMDGVHPDMDTCALIDYKGHLILDTVDCTNPN GGRLPIGVDMMLSDFAGGASGFPMTFSGGKYTEEWKAEFVKRERRKLLYYKMQQVRDVAP TVYCPFAGYFVEAHPSDHYIRSTNTKNDPDALNALINKYSPNIKTWSPSPGAVLDLKKAI QGDRDFITDPPRGTQKFKDSWDFEKYVNAINKNIEEEIFSYPEWIQFYYKWTGFKNYNLV IRMVERDDDFCPVVGGYDFMVDFVGEEPTFPTERPAREHSYLEMENRIGVHRETVRQGLF WDDLYIGFNNRISREPDTFHYLFWNHMQILLPRTDPDWEGFLRDMKTEGAPQKAIWNPSQ ATPAVEAKDPSSDSKDSATKPGTHWNYERLLRPLGIVVALVGVGVAIWKSESK .
3.1. Enzyme Function
Cytidine monophosphate-N-acetylneuraminic acid hydroxylase is an NADH-dependent enzyme that catalyzes the conversion of CMP-N-acetylneuraminic acid to CMP-N-glycolylneuraminic acid. This reaction is crucial for the synthesis of Neu5Gc, a sialic acid form prevalent in echinoderms like Asterias rubens .
3.2. Comparison with Mammalian Enzymes
Unlike mammalian hydroxylases, which are primarily soluble, the echinoderm enzyme is predominantly membrane-bound and requires specific conditions for optimal activity, such as the presence of 100 mM NaCl . The interaction with cytochrome b5 and cytochrome b5 reductase is essential for its catalytic activity, similar to mammalian enzymes .
3.3. Potential Applications
The recombinant enzyme can be used in biochemical studies to understand sialic acid metabolism and its role in echinoderms. It may also serve as a tool for producing Neu5Gc, which has implications in glycoconjugate synthesis and potentially in biomedical applications.
4.2. Comparison of Echinoderm and Mammalian Hydroxylases
| Feature | Echinoderm Hydroxylase | Mammalian Hydroxylase |
|---|---|---|
| Localization | Predominantly membrane-bound | Primarily soluble |
| Optimal Conditions | 100 mM NaCl required | No specific salt requirement |
| Interaction | Requires cytochrome b5 and b5 reductase | Similar interaction with cytochrome b5 and b5 reductase |
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
Sialic acids are carbohydrate chain components of glycoconjugates, playing crucial roles in cell-cell recognition and cell-pathogen interactions. This recombinant Asterias rubens cytidine monophosphate-N-acetylneuraminic acid hydroxylase (cnh) catalyzes the conversion of CMP-N-acetylneuraminic acid (CMP-Neu5Ac) to its hydroxylated derivative, CMP-N-glycolylneuraminic acid (CMP-Neu5Gc), a sialic acid prevalent on the surface of many cells.
