Recombinant Azoarcus sp. NADH-quinone oxidoreductase subunit K (nuoK)
Description
Production and Purification
Recombinant NuoK is produced in E. coli expression systems, followed by affinity chromatography . Critical parameters include:
-
Expression Vector: Optimized for high-yield soluble protein .
-
Storage Buffer: Tris-based buffer with 50% glycerol, pH adjusted for stability .
Reconstitution Guidelines:
-
Lyophilized protein is reconstituted in sterile deionized water (0.1–1.0 mg/mL) .
-
Glycerol (5–50%) is added for long-term storage at -20°C/-80°C .
Biochemical Applications
-
Respiratory Studies: Used to dissect electron transport mechanisms in β-proteobacteria .
-
Drug Target Screening: NDH-1 is a potential target for antimicrobial agents due to its role in pathogen bioenergetics .
-
Enzyme Engineering: Serves as a template for optimizing quinone reductase activity in synthetic biology .
Stability and Handling
Research Gaps
-
Structural Data: No high-resolution structures for Azoarcus NuoK limit mechanistic insights.
-
Kinetic Parameters: Michaelis constants (Kₘ) for NADH/quinones remain uncharacterized.
Product Specs
Please note: We will prioritize shipping the format currently in stock. However, if you have specific requirements for the format, please indicate them in your order. We will prepare the product according to your request.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance as additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: azo:azo1406
STRING: 62928.azo1406
