Recombinant Bacillus amyloliquefaciens Biotin synthase (bioB)
Description
Introduction to Recombinant Bacillus amyloliquefaciens Biotin Synthase (bioB)
Recombinant Bacillus amyloliquefaciens biotin synthase (bioB) is an engineered enzyme derived from the bioB gene, which plays a crucial role in the biosynthesis of biotin. Biotin is a vital vitamin (B7) essential for various metabolic processes, including fatty acid synthesis, gluconeogenesis, and amino acid metabolism. The bioB gene encodes the enzyme biotin synthase, responsible for converting dethiobiotin into biotin in the final step of biotin biosynthesis.
While specific research on recombinant Bacillus amyloliquefaciens biotin synthase (bioB) is limited, understanding its function and potential applications can be inferred from studies on related organisms and biotin synthesis pathways.
Biotin Synthesis Pathway
The biotin biosynthesis pathway involves several enzymes encoded by genes such as bioA, bioB, bioC, bioD, bioF, and bioH. In Bacillus subtilis, these genes are clustered in a single operon, facilitating coordinated regulation of biotin production . The pathway starts with the synthesis of pimelic acid, which is then converted into pimeloyl-CoA by BioW. This intermediate is further processed to form dethiobiotin, which is finally converted to biotin by biotin synthase (BioB) .
Role of Biotin Synthase (BioB)
Biotin synthase (BioB) is a radical SAM enzyme that catalyzes the conversion of dethiobiotin to biotin. This enzyme requires a radical SAM cofactor and a reducing agent, typically S-adenosylmethionine (SAM), to facilitate the insertion of sulfur into dethiobiotin . In some bacteria, like Mycobacterium tuberculosis, BioB requires an auxiliary protein for activity .
Recombinant Expression and Applications
Recombinant expression of biotin synthase in microorganisms like Bacillus amyloliquefaciens could enhance biotin production and potentially improve the organism's metabolic capabilities. Bacillus amyloliquefaciens is known for its versatility in producing various enzymes and secondary metabolites, making it an attractive host for metabolic engineering .
Table 1: Key Enzymes in Biotin Biosynthesis
| Enzyme | Function | Organism |
|---|---|---|
| BioA | Converts pimeloyl-CoA to 7-keto-8-aminopelargonic acid | Bacillus subtilis, Escherichia coli |
| BioB | Converts dethiobiotin to biotin | Bacillus subtilis, Escherichia coli, Mycobacterium tuberculosis |
| BioC | Converts 7-keto-8-aminopelargonic acid to 7,8-diaminopelargonic acid | Bacillus subtilis, Escherichia coli |
| BioD | Converts 7,8-diaminopelargonic acid to dethiobiotin | Bacillus subtilis, Escherichia coli |
| BioF | Converts pimeloyl-CoA to 8-amino-7-oxononanoate | Bacillus subtilis, Escherichia coli |
| BioH | Converts 8-amino-7-oxononanoate to 7-keto-8-aminopelargonic acid | Bacillus subtilis, Escherichia coli |
| BioW | Converts pimelic acid to pimeloyl-CoA | Bacillus subtilis |
Research Findings and Potential Applications
While specific studies on recombinant Bacillus amyloliquefaciens biotin synthase (bioB) are not available, research on related biotin synthesis pathways and enzymes provides valuable insights. Enhancing biotin production in Bacillus amyloliquefaciens could improve its metabolic capabilities, potentially increasing the yield of secondary metabolites like lipopeptides, which have applications in agriculture and biotechnology .
Table 2: Potential Applications of Enhanced Biotin Production
| Application | Description |
|---|---|
| Metabolic Engineering | Improving metabolic pathways for enhanced production of secondary metabolites like lipopeptides and surfactins. |
| Agricultural Biotechnology | Utilizing engineered strains for biocontrol and plant growth promotion. |
| Industrial Biotechnology | Enhancing production of enzymes and bioactive compounds for various industrial applications. |
Product Specs
Target Background
This enzyme catalyzes the conversion of dethiobiotin (DTB) to biotin through the radical-based insertion of a sulfur atom into dethiobiotin.
