Recombinant Bacillus anthracis UPF0295 protein BAA_0600 (BAA_0600)
Description
Expression and Purification
BAA_0600 is expressed in E. coli using a His-tagged expression vector, enabling affinity chromatography purification. The protein is lyophilized and provided in a sterile, ready-to-use format. Key specifications include:
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Form: Lyophilized powder.
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Stability: Stable at -20°C/-80°C when stored properly.
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Handling Notes: Repeated freezing/thawing is discouraged; store working aliquots at 4°C for short-term use .
Functional Insights and Pathways
While BAA_0600 is annotated as a UPF0295 family protein, its specific biochemical functions remain poorly characterized in available literature. Limited data suggest potential involvement in pathways related to B. anthracis survival or pathogenicity, though no direct functional studies (e.g., enzymatic activity or interaction networks) are reported.
Key Observations:
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Pathway Involvement: No validated pathways are listed in current databases .
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Interacting Proteins: No experimentally confirmed interactions are documented .
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Role in Anthrax: Unlike protective antigen (PA) or spore proteins (e.g., BclA, BxpB), BAA_0600 is not directly linked to toxin production or immune evasion mechanisms .
Research Applications
BAA_0600 serves primarily as a research reagent for studying B. anthracis protein interactions, spore biology, or pathogenicity mechanisms. Potential applications include:
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Structural Studies: Investigating the UPF0295 family’s role in bacterial physiology.
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Antigen Screening: Testing BAA_0600 as a candidate in vaccine or diagnostic assays (though no efficacy data exists).
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Protein-Protein Interactions: Identifying binding partners using co-IP or pull-down assays.
Limitations and Gaps
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Functional Data: No published studies describe BAA_0600’s enzymatic activity, localization, or role in B. anthracis infection.
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Vaccine Relevance: Unlike PA or spore antigens (e.g., BxpB), BAA_0600 is not implicated in immune protection or toxin neutralization .
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Experimental Validation: Further studies are required to elucidate its biological significance.
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes if needed. We will accommodate your request whenever possible.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is specifically requested and agreed upon in advance. Additional fees apply for dry ice shipping.
Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
If a specific tag type is required, please inform us, and we will prioritize its use in production.
Target Background
KEGG: bai:BAA_0600
