Recombinant Bacillus cereus Cardiolipin synthase 2 (cls2)
Description
Introduction to Recombinant Bacillus cereus Cardiolipin Synthase 2 (cls2)
Recombinant Bacillus cereus Cardiolipin Synthase 2 (cls2) is a genetically engineered enzyme derived from the bacterium Bacillus cereus. Cardiolipin synthases are crucial for the synthesis of cardiolipin, a phospholipid essential for maintaining the structural integrity and function of bacterial membranes. In bacteria, cardiolipin plays a significant role in membrane stability, particularly under stress conditions such as high temperatures or acidic environments.
Function and Importance of Cardiolipin Synthase
Cardiolipin synthases catalyze the final step in cardiolipin biosynthesis, combining two phosphatidylglycerol molecules to form cardiolipin. This process is vital for bacterial survival and virulence, as cardiolipin helps modulate membrane fluidity and supports the function of membrane-bound proteins. In Bacillus cereus, cardiolipin synthase 2 (cls2) is involved in cardiolipin accumulation during stationary phase growth and under specific stress conditions.
Characteristics of Recombinant Bacillus cereus Cardiolipin Synthase 2
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Expression and Source: Recombinant Bacillus cereus Cardiolipin Synthase 2 is typically expressed in Escherichia coli (E. coli), a common host for recombinant protein production due to its well-understood genetics and ease of manipulation.
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Purity and Form: The recombinant enzyme is often purified to a high degree, typically greater than 85% as determined by SDS-PAGE, and is available in a lyophilized powder form.
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Applications: It is used in research settings for studying bacterial membrane biology, cardiolipin synthesis pathways, and the role of cardiolipin in bacterial stress responses.
Research Findings and Data
While specific data on recombinant Bacillus cereus Cardiolipin Synthase 2 is limited, studies on cardiolipin synthases in general highlight their importance in bacterial physiology. For instance, in Staphylococcus aureus, cardiolipin synthases (cls1 and cls2) are crucial for modulating virulence and adapting to host environments by influencing sensor kinase activities .
Table: Characteristics of Recombinant Cardiolipin Synthases
| Protein | Species | Expression Host | Purity | Form |
|---|---|---|---|---|
| Cls1 | Bacillus cereus | E. coli | >90% | Lyophilized powder |
| Cls2 | Staphylococcus aureus | E. coli | >90% | Lyophilized powder |
| Cls2 | Bacillus cereus | Not specified | ≥85% | Not specified |
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.
Note: Our proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The specific tag type is determined during production. If you require a particular tag, please specify it to enable preferential development.
Target Background
Function: Catalyzes the reversible transfer of phosphatidyl groups between phosphatidylglycerol molecules, resulting in the formation of cardiolipin (CL, diphosphatidylglycerol) and glycerol.
KEGG: bce:BC1191
STRING: 226900.BC1191
