Recombinant Bacillus cereus UPF0059 membrane protein BCE_5451 (BCE_5451)
Description
Production and Handling
BCE_5451 is produced via recombinant expression in E. coli, followed by purification using His-tag affinity chromatography. Critical handling parameters include:
3.1. Membrane Protein Studies
BCE_5451 is used to investigate Bacillus cereus membrane biology, particularly in spore and vegetative cell membranes. While not directly studied in spore inner membrane proteomes , its classification as a UPF0059 protein aligns with broader research on Bacillus membrane proteins:
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Spore Inner Membrane: Enriched with germinant receptors and spore-specific proteins, though BCE_5451 is not explicitly identified in these fractions .
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Vegetative Cell Membrane: Dominated by transporters, receptors, and motility-related proteins, which may share functional parallels with BCE_5451 .
3.2. Diagnostic and Detection Tools
Recombinant BCE_5451 is utilized in ELISA kits for detecting Bacillus cereus or studying UPF0059 family interactions. These assays leverage the protein’s His-tag for antibody binding .
Functional Hypotheses
While no direct studies confirm BCE_5451’s function, its association with mntP (manganese efflux pump) suggests roles in:
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Metal Ion Transport: Regulating intracellular manganese levels, critical for enzymatic activity and oxidative stress resistance.
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Sensory Transduction: Potentially sensing environmental cues (e.g., pH, metal availability) via membrane localization.
Comparative Context
BCE_5451 is part of a larger Bacillus cereus membrane proteome, which includes:
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Spore-Specific Proteins: Germinant receptors (e.g., SleB, HtrC orthologs) and spore coat components .
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Vegetative Cell Proteins: Flagellins (linked to motility ), transporters, and division/motility factors .
| Protein Type | Spore Inner Membrane | Vegetative Cell Membrane |
|---|---|---|
| Transporters | Limited (e.g., glucose/fructose) | Abundant (diverse substrates) |
| Motility Factors | None | Flagellins, chemotaxis sensors |
| Germination Receptors | Present (e.g., SleB, HtrC-like) | Absent |
Challenges and Gaps
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Functional Characterization: No studies directly link BCE_5451 to manganese efflux or sensory pathways.
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Strain-Specific Variability: Bacillus cereus strains exhibit diverse genetic backgrounds (e.g., toxin gene prevalence) , potentially influencing BCE_5451’s role.
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Structural Insights: The protein’s 3D structure and membrane topology remain uncharacterized.
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it in your order notes, and we will prepare the product accordingly.
Note: All proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance, as additional fees will apply.
Generally, liquid form has a shelf life of 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: bca:BCE_5451
