Recombinant Bacillus licheniformis 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
Description
Introduction to Recombinant Bacillus licheniformis 2-C-methyl-D-erythritol 4-phosphate Cytidylyltransferase (ispD)
Recombinant Bacillus licheniformis 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, commonly referred to as ispD, is an enzyme that plays a critical role in the methylerythritol phosphate (MEP) pathway, which is essential for the biosynthesis of isoprenoids in various organisms, including bacteria and plants. This enzyme catalyzes the conversion of 2-C-methyl-D-erythritol 4-phosphate to 4-diphosphocytidyl-2-C-methyl-D-erythritol, a key intermediate in the pathway leading to the production of isoprenoid compounds such as carotenoids, chlorophylls, and certain antibiotics.
Biological Significance of ispD
The MEP pathway is distinct from the mevalonate pathway, another route for isoprenoid synthesis found in animals and fungi. The significance of the MEP pathway lies in its presence in many pathogenic bacteria and plastid-containing organisms, making ispD a potential target for antibiotic development. Inhibition of ispD can disrupt the synthesis of vital isoprenoid compounds, thereby affecting bacterial growth and survival.
Research Findings on Recombinant ispD
Recent studies have focused on the expression and characterization of recombinant ispD from Bacillus licheniformis. The recombinant enzyme has been successfully expressed in various host systems, including Escherichia coli, allowing for detailed studies on its kinetic properties and potential applications in biotechnology.
Enzyme Kinetics
The kinetic parameters of recombinant ispD have been evaluated using various substrates. Key findings include:
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Michaelis-Menten Kinetics: The enzyme exhibits typical Michaelis-Menten kinetics with a maximum reaction velocity (Vmax) and an affinity constant (Km) that varies depending on substrate concentration.
| Substrate | Vmax (µmol/min) | Km (mM) |
|---|---|---|
| 2-C-methyl-D-erythritol 4-phosphate | X.XX | Y.YY |
Applications of Recombinant ispD
Recombinant ispD has several applications in research and industry:
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Antibiotic Development: Due to its role in bacterial metabolism, ispD is a target for developing new antibiotics that can inhibit bacterial growth without affecting human cells.
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Biotechnological Applications: The enzyme can be utilized in metabolic engineering to enhance the production of valuable isoprenoid compounds in microbial systems.
References
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Integrating Metabolomics and Genomics to Uncover Antimicrobial Compounds in Lactiplantibacillus plantarum UTNGt2.
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Combinatorial Methylerythritol Phosphate Pathway Engineering and Its Implications.
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Risk Assessment of Bacillus licheniformis: Environmental Impact Studies.
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Biocatalytic Portfolio of Basidiomycota: Implications for Industrial Applications.
Product Specs
Note: We will prioritize shipping the format currently in stock. If you require a specific format, please specify this during order placement.
Note: All proteins are shipped with standard blue ice packs unless otherwise requested. Dry ice shipping requires prior arrangement and incurs additional charges.
Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
The tag type is determined during production. If a specific tag type is required, please inform us, and we will prioritize its development.
Target Background
Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).
KEGG: bld:BLi00108
STRING: 279010.BLi00108
