Recombinant Bacillus licheniformis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: We will prioritize shipment of the format currently in stock. If you require a specific format, please specify this during order placement.
Note: We will prioritize shipment of the format currently in stock. If you require a specific format, please specify this during order placement.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a guideline.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during the manufacturing process.
The tag type is determined during production. If you require a specific tag type, please inform us, and we will prioritize its development.
The tag type is determined during production. If you require a specific tag type, please inform us, and we will prioritize its development.
Synonyms
gatB; BLi00732; BL00602; Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.-
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-476
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Target Names
gatB
Target Protein Sequence
MNFETVIGLE VHVELKTKSK IFSSSPTPFG AAANTQTSVI DLGYPGVLPV LNKEAVNFAM
KAAMALNCEI ATDTKFDRKN YFYPDNPKAY QISQFDKPIG ENGWIEIEVG GKTKRIGITR
LHLEEDAGKL THTGDGYSLV DYNRQGTPLV EIVSEPDIRT PEEAYAYLEK LKSIIQYTGV
SDCKMEEGSL RCDANISLRP IGQEEFGTKT ELKNLNSFAF VQKGLEHEEK RQAQVLLSGG
VIQQETRRYD EASKTTILMR VKEGSDDYRY FPEPDLVELY IDDEWKQRVK DSIPELPDER
RKRYIEELGL PAYDAKVLTL TKEMSDFFEA AVEKGADAKL ASNWLMGEVS AYLNAQQKEL
ADVELTPEGL AGLVKLIEKG TISSKIAKKV FKELIEKGGD AEKIVKEKGL VQISDEATLR
KLVTEALDNN PQSIEDFKNG KDRAIGFLVG QIMKASKGQA NPPMVNKLLL EEINKR
Uniprot No.
Target Background
Function
This protein facilitates the formation of correctly charged Asn-tRNA(Asn) and Gln-tRNA(Gln) by transamidating misacylated Asp-tRNA(Asn) and Glu-tRNA(Gln). This process is crucial in organisms lacking either or both asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction requires glutamine and ATP, proceeding through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) intermediate.
Database Links
KEGG: bld:BLi00732
STRING: 279010.BLi00732
Protein Families
GatB/GatE family, GatB subfamily
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