Recombinant Bacillus licheniformis Bifunctional enzyme MtnB/MtnX (mtnB/mtnX)

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In Stock
Cat. No.
BT2434765
Source
Yeast
Synonyms
mtnB/mtnX; BLi01516; BL03541; Bifunctional enzyme MtnB/MtnX [Includes: Methylthioribulose-1-phosphate dehydratase; MTRu-1-P dehydratase; EC 4.2.1.109); 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase; HK-MTPenyl-1-P phosphatase; EC 3.1.3.87)]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434765
Source
E.coli
Synonyms
mtnB/mtnX; BLi01516; BL03541; Bifunctional enzyme MtnB/MtnX [Includes: Methylthioribulose-1-phosphate dehydratase; MTRu-1-P dehydratase; EC 4.2.1.109); 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase; HK-MTPenyl-1-P phosphatase; EC 3.1.3.87)]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434765
Source
E.coli
Synonyms
mtnB/mtnX; BLi01516; BL03541; Bifunctional enzyme MtnB/MtnX [Includes: Methylthioribulose-1-phosphate dehydratase; MTRu-1-P dehydratase; EC 4.2.1.109); 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase; HK-MTPenyl-1-P phosphatase; EC 3.1.3.87)]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434765
Source
Baculovirus
Synonyms
mtnB/mtnX; BLi01516; BL03541; Bifunctional enzyme MtnB/MtnX [Includes: Methylthioribulose-1-phosphate dehydratase; MTRu-1-P dehydratase; EC 4.2.1.109); 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase; HK-MTPenyl-1-P phosphatase; EC 3.1.3.87)]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434765
Source
Mammalian cell
Synonyms
mtnB/mtnX; BLi01516; BL03541; Bifunctional enzyme MtnB/MtnX [Includes: Methylthioribulose-1-phosphate dehydratase; MTRu-1-P dehydratase; EC 4.2.1.109); 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase; HK-MTPenyl-1-P phosphatase; EC 3.1.3.87)]
Purity
>85% (SDS-PAGE)
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Product Specs

Form
Lyophilized powder
Note: We will prioritize shipping the format currently in stock. If you require a specific format, please specify this in your order notes, and we will fulfill your request to the best of our ability.
Lead Time
Delivery times vary depending on the purchase method and location. Please contact your local distributor for precise delivery estimates.
Note: All proteins are shipped on standard blue ice packs. Dry ice shipping is available upon request, but will incur additional charges. Please contact us in advance to arrange this.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50%, and this may serve as a helpful reference.
Shelf Life
Shelf life depends on several factors including storage conditions, buffer composition, temperature, and the inherent stability of the protein. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type will be determined during manufacturing.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its inclusion.
Synonyms
mtnB/mtnX; BLi01516; BL03541; Bifunctional enzyme MtnB/MtnX [Includes: Methylthioribulose-1-phosphate dehydratase; MTRu-1-P dehydratase; EC 4.2.1.109); 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase; HK-MTPenyl-1-P phosphatase; EC 3.1.3.87)]
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-427
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Target Names
mtnB/mtnX
Target Protein Sequence
MRKPLIICDF DGTITTNDNI ISIMKQFAPE EWTALKDGVL SKDISIRDGV GRMFNLLPAS LKEDITAYVL KQAETRPGFK EFVSFLDEKG LPFYVVSGGM DFFVYPLLEG IVGKDRIYCN EAAFTSRNIE IRWPYPCDGG CGNDCGCCKP SIIRRLKGRD DFVVMIGDSV TDVEAAKCSD LCIARDYLLR ECEELGLKHA AFGDFRDVRR ILEETAEVKE WMSEQKRQEL AEVKKELAER DWFPATSGNL SIKVSEDPLR FLITASGKDK RKETEEDFLL ADEEGRPAET GHALKPSAET LLHTYVYQHT NAGCCLHVHT VDNNVISELY AKEKQVTFRG QEIIKALGLW EEHAEVTVPI IENSAHIPDL AADFAGHLSG DSGAVLIRSH GITVWGKTAF EAKRMLEAYE FLFSWHLKLK ALQAYHV
Uniprot No.
Q65KJ7

Target Background

Function

This bifunctional enzyme catalyzes two reactions: the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) to 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P), and the dephosphorylation of 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P) to 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).

Database Links

KEGG: bld:BLi01516

STRING: 279010.BLi01516

Protein Families
HAD-like hydrolase superfamily, MtnX family; Aldolase class II family, MtnB subfamily

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