Recombinant Bacillus licheniformis Ribosomal RNA large subunit methyltransferase N (rlmN)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50%, which may serve as a guideline.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and the protein's inherent stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type is determined during production. If a specific tag type is required, please inform us; we will prioritize its development.
The tag type is determined during production. If a specific tag type is required, please inform us; we will prioritize its development.
Synonyms
rlmN; BLi01796; BL02300; Probable dual-specificity RNA methyltransferase RlmN; EC 2.1.1.192; 23S rRNA; adenine(2503)-C(2))-methyltransferase; 23S rRNA m2A2503 methyltransferase; Ribosomal RNA large subunit methyltransferase N; tRNA; adenine(37)-C(2))-methyltransferase; tRNA m2A37 methyltransferase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-361
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Target Names
rlmN
Target Protein Sequence
MESKQKVRKE LRTERPSIYS FEIEELKSWL SENGEKPFRA AQIFEWLYEK RVTSFEKMTN
LSKELRTKLN EHFVLTTLKT AVKQTSQDGT MKFLFELHDG YTIETVLMRH EYGNSVCVTT
QVGCRIGCTF CASTLGGLKR NLEAGEIVAQ VVKVQQALDE TDERVSSIVI MGIGEPFDNF
QEMLAFLKIV NHDKGLNIGA RHITVSTSGI IPKIYEFADE KLQINFAISL HAPNTEIRSR
LMPINRAYKL PDLMKAVDYY IKKTGRRVTF EYGLFGGVND QVEHAEELAE LLKGIKCHVN
LIPVNYVPER DYVRTPKEQI FAFEKTLKSH GVNVTIRREQ GHDIDAACGQ LRAKERQEET
R
Uniprot No.
Target Background
Function
Specifically methylates adenine at position 2 of 23S rRNA (adenine 2503) and adenine at position 2 of various tRNAs (adenine 37).
Database Links
KEGG: bld:BLi01796
STRING: 279010.BLi01796
Protein Families
Radical SAM superfamily, RlmN family
Subcellular Location
Cytoplasm.
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