Recombinant Bacillus licheniformis Ribosome maturation factor RimM (rimM)

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Cat. No.
BT2434085
Source
Yeast
Synonyms
rimM; BLi01822; BL05160; Ribosome maturation factor RimM
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434085
Source
E.coli
Synonyms
rimM; BLi01822; BL05160; Ribosome maturation factor RimM
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434085
Source
E.coli
Synonyms
rimM; BLi01822; BL05160; Ribosome maturation factor RimM
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434085
Source
Baculovirus
Synonyms
rimM; BLi01822; BL05160; Ribosome maturation factor RimM
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434085
Source
Mammalian cell
Synonyms
rimM; BLi01822; BL05160; Ribosome maturation factor RimM
Purity
>85% (SDS-PAGE)
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Description

Genetic Engineering Strategies in B. licheniformis

Recombinant RimM production in B. licheniformis leverages advanced genetic tools:

  • Thermosensitive plasmid systems: pUB-MazF enables efficient plasmid curing via MazF endoribonuclease, facilitating stable chromosomal integration of rimM .

  • Promoter systems:

    • Rhamnose-inducible promoter (P<sub>rha</sub>): Enhances recombination efficiency by 10<sup>5</sup>-fold when induced with 0–20 g/L rhamnose .

    • Bacitracin synthase promoter (P<sub>bacA</sub>): Strong endogenous promoter used for high-yield protein expression .

Case study: A B. licheniformis strain with multi-copy chromosomal integration of amyS (α-amylase gene) achieved 50 U/mL enzyme activity in bioreactors , suggesting similar strategies could amplify rimM expression.

Functional Role in Ribosome Maturation

RimM ensures proper 16S rRNA processing and 30S subunit assembly:

  • Binds helix 33b of 16S rRNA to enable S19 incorporation .

  • Prevents accumulation of unprocessed 17S rRNA precursors .

  • In Arabidopsis thaliana chloroplasts, RimM homologs coordinate with RsmD methyltransferase for rRNA methylation (e.g., m²G915) and translation fidelity .

Impact on cellular physiology:

  • B. licheniformis ribosome content varies with growth rate (12,500–92,000 ribosomes/cell) .

  • RimM deficiency disrupts ribosome assembly, impairing protein synthesis and stress adaptation .

Biotechnological Applications

Recombinant RimM can enhance B. licheniformis as an industrial enzyme producer:

  • Strain optimization: Overexpression of RimM may accelerate ribosome assembly, boosting yields of:

    • α-Amylase (17.6 mg/mL achieved via plasmid pHY-amyL) .

    • Antimicrobial peptides (e.g., lichenicin, bacitracin) .

  • Probiotic engineering: RimM-assisted ribosome biogenesis could improve stress tolerance in probiotic strains like B. licheniformis NXU98 .

Research Gaps and Future Directions

  • Structural studies: No B. licheniformis RimM structures are available; homology modeling using T. thermophilus data (PDB: 2D9X) is needed.

  • Metabolic burden: High RimM expression may require balancing with toxin-antitoxin systems (e.g., MazF/MazE) .

  • Antimycobacterial potential: RimM’s role in ribosome maturation could inform novel tuberculosis therapies via B. licheniformis-derived antimicrobials .

Product Specs

Form
Lyophilized powder
Lead Time
Delivery times vary depending on the purchase method and location. Please contact your local distributor for specific delivery timelines.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, which can serve as a reference.
Shelf Life
Shelf life depends on several factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is recommended for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
rimM; BLi01822; BL05160; Ribosome maturation factor RimM
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-174
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Target Names
rimM
Target Protein Sequence
MLKQWFNVGK IVNTHGVKGE VRVISRTDFP EERYKPGNAL YLFLEGKDEP VQVTVSAHRL HKQFHLLQFK EVETLTEAEK LKNALIKVPE DQLSQLAEDE YYFHEIIGCD VFSEDGDLIG KVKEILTPGA NDVWVVARPG KKDALIPYID AVVKEINVAD KTIKIHIMEG LLDE
Uniprot No.
Q65JP7

Target Background

Function
RimM is an accessory protein crucial for the final assembly of the 30S ribosomal subunit, likely contributing to the head region's formation. It possibly interacts with ribosomal protein S19 and is essential for efficient 16S rRNA processing. RimM appears necessary both before and after RbfA during 16S rRNA maturation. It exhibits affinity for free 30S ribosomal subunits but not for 70S ribosomes.
Database Links

KEGG: bld:BLi01822

STRING: 279010.BLi01822

Protein Families
RimM family
Subcellular Location
Cytoplasm.

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