Recombinant Bacillus subtilis Sporulation protein yjcA (yjcA)
Description
Background and Function
Research Findings
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Expression and Regulation: Sporulation genes, including those like yjcA, are tightly regulated and expressed at specific stages of sporulation. The expression of these genes is often dependent on specific sigma factors, which are RNA polymerase subunits that direct transcription to different promoters .
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Mutational Studies: Mutational studies on related proteins have shown that disruptions in sporulation genes can lead to defects in spore formation and resistance. For example, mutations in yjcC result in spores with reduced resistance to lysozyme and heat .
Recombinant Production
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Recombinant Expression: Recombinant yjcA is produced through genetic engineering techniques where the gene encoding yjcA is inserted into a suitable expression vector and expressed in a host organism, often B. subtilis itself or other bacteria like E. coli.
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Applications: Recombinant proteins like yjcA can be used for research purposes, such as studying the sporulation process or developing new biotechnological applications related to spore formation and resistance.
Comparison with Other Sporulation Proteins
| Protein | Function | Expression Stage |
|---|---|---|
| yjcA | Unknown, involved in sporulation | Specific stage of sporulation |
| yjcC (spoVIF) | Involved in spore coat assembly | Stage VI of sporulation |
| SpoIIIJ | Required for spore formation, involved in membrane protein biogenesis | Specific stage of sporulation |
References
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ChemicalBook - Recombinant Bacillus subtilis proteins.
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Kuwana et al. (2002) - Bacillus subtilis spoVIF (yjcC) gene involved in coat assembly and resistance.
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Structure-Function Studies of the Bacillus subtilis Ric Proteins (2019) - Although not directly related to yjcA, this study highlights the complexity of protein functions in B. subtilis.
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SpoIIIJ and YqjG Function in Membrane Protein Biogenesis (2009) - Demonstrates the role of other proteins in B. subtilis related to membrane biogenesis.
Product Specs
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The tag type is determined during the production process. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
Involved in sporulation.
KEGG: bsu:BSU11790
STRING: 224308.Bsubs1_010100006511
