Recombinant Bacillus subtilis Uncharacterized membrane protein ybgB (ybgB)
Description
Regulatory Context and Genomic Organization
ybgB is transcriptionally regulated by the extracytoplasmic function (ECF) sigma factor σY (sigY) . Key regulatory features include:
The ybgA-gamAP synton, unique to B. subtilis, includes ybgB but lacks clear functional overlap with amino sugar metabolism genes (gamAP) . Microarray studies show ybgB expression increases 71-fold in σY-activated strains, indicating direct σY-dependent regulation .
Membrane Localization and Potential Roles
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Transport/Regulation: ybgB’s hydrophobicity and operon context suggest involvement in membrane-associated transport or stress response pathways .
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Secondary Metabolism: While not directly linked to known B. subtilis secondary metabolites (e.g., pigments ), its regulation by σY—a stress-responsive sigma factor—hints at adaptive roles .
Experimental Insights
Recombinant Protein Production
The recombinant ybgB protein is commercially available for research, enabling:
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Structural Studies: NMR or X-ray crystallography to resolve membrane topology.
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ELISA: Detection of ybgB-specific antibodies or interactions .
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Functional Screens: Testing transport activity or regulatory interactions with σY/YxlD/YxlE .
Future Research Directions
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Functional Characterization:
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Heterologous expression in E. coli to test transport activity.
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Knockout studies in B. subtilis to assess phenotypic effects.
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Structural Elucidation:
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Cryo-EM or NMR to determine membrane topology and interactions.
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Regulatory Interactions:
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement, and we will fulfill your request.
Note: All our proteins are shipped with standard blue ice packs. If dry ice shipping is required, please inform us in advance, as additional fees will apply.
Generally, the shelf life of liquid forms is 6 months at -20°C/-80°C. For lyophilized forms, the shelf life is 12 months at -20°C/-80°C.
Target Background
KEGG: bsu:BSU02380
STRING: 224308.Bsubs1_010100001313
