Recombinant Bacillus subtilis Uncharacterized membrane protein yczE (yczE)
Description
Functional Context in Bacillus subtilis
While yczE remains uncharacterized, homologs in B. subtilis (e.g., SpoIIIJ and YqjG) are implicated in membrane protein biogenesis:
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Role in Membrane Insertion: SpoIIIJ and YqjG facilitate Sec-dependent and -independent insertion of cytochrome oxidase and ATP synthase subunits, suggesting yczE may participate in similar processes .
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Association with ATP Synthase: SpoIIIJ/YqjG copurify with the F1Fo ATP synthase complex, hinting at a potential late-stage assembly role for related proteins .
3.1. Expression Platforms
B. subtilis is a preferred host for recombinant protein production due to:
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GRAS/QPS Status: Safe for industrial and biomedical applications .
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Secretion Efficiency: Engineered strains (e.g., WB800N) optimize yields .
Table 1: Key B. subtilis Expression Features
3.2. yczE-Specific Production
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Cloning Vectors: pHT43 and pUC57 plasmids are commonly used for heterologous expression in B. subtilis .
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Storage: Recombinant yczE is stable at -20°C to -80°C, with glycerol (50%) recommended for long-term storage .
Research Gaps and Future Directions
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Functional Annotation: No direct studies on yczE’s biological role exist; homology modeling or knockout studies could elucidate its function.
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Optimization: Screening yczE with diverse promoters (e.g., P43, Pgrac) or signal peptides may improve expression .
Implications for Biotechnology
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them in your order notes. We will then prepare your order accordingly.
Note: All of our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance. Additional fees may apply.
Generally, liquid formulations have a shelf life of 6 months at -20°C/-80°C. Lyophilized formulations have a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: bsu:BSU03580
STRING: 224308.Bsubs1_010100002033
